The Minor Matrix Protein VP24 from Ebola Virus Lacks Direct Lipid-Binding Properties. Viral protein 24 (VP24) from Ebola virus (EBOV) was first acknowledged as a minor matrix protein that associates with mobile membranes. Nonetheless, more moderen research make clear its roles in inhibiting viral genome transcription and replication, facilitating nucleocapsid meeting and transport, and interfering with immune responses in host cells via downregulation of interferon (IFN)-activated genes.
Thus, whether or not VP24 is a peripheral protein with lipid-binding potential for matrix layer recruitment has not been explored. Right here, we examined the lipid-binding potential of VP24 with quite a lot of lipid-binding assays. The outcomes indicated that VP24 lacked the power to affiliate with lipids examined no matter VP24 posttranslational modifications.
We additional display that the presence of the EBOV main matrix protein VP40 didn’t promote VP24 membrane affiliation in vitro or in cells. Additional, no protein-protein interactions between VP24 and VP40 had been detected by co-immunoprecipitation. Confocal imaging and mobile membrane fractionation analyses in human cells advised VP24 didn’t particularly localize on the plasma membrane internal leaflet.
General, we offer proof that EBOV VP24 will not be a lipid-binding protein and its presence within the viral matrix layer is probably going not depending on direct lipid interactions.
Key phrases: Ebola virus; VP24; filovirus; lipid binding; matrix protein.
Anticancer and Antiviral Properties of Cardiac Glycosides: A Overview to Discover the Mechanism of Actions Cardiac glycosides (CGs) have an extended historical past of treating cardiac ailments. Nonetheless, current experiences have advised that CGs additionally possess anticancer and antiviral actions. The first mechanism of motion of those anticancer brokers is by suppressing the Na+ /ok+ -ATPase by reducing the intracellular Okay+ and growing the Na+ and Ca2+ . Moreover, CGs had been recognized to behave as inhibitors of IL8 manufacturing, DNA topoisomerase I and II, anoikis prevention and suppression of a number of goal genes chargeable for the inhibition of most cancers cell proliferation.
Furthermore, CGs had been reported to be efficient towards a number of DNA and RNA viral species equivalent to influenza, human cytomegalovirus, herpes simplex virus, coronavirus, tick-borne encephalitis (TBE) virus and Ebola virus. CGs had been reported to suppress the HIV-1 gene expression, viral protein translation and alters viral pre-mRNA splicing to inhibit the viral replication.
Thus far, 4 CGs (Anvirzel, UNBS1450, PBI05204 and digoxin) had been in medical trials for his or her anticancer exercise . This evaluate encapsulates the present data about CGs as anticancer and antiviral medication in isolation and together with another medication to boost their effectivity. Additional research of this class of biomolecules are mandatory to find out their attainable inhibitory position in most cancers and viral ailments.
zebov-detection
Key phrases: anticancer; antiviral; autophagy; biomolecules; cardiac glycosides; preclinical trials; signaling pathway; viral protein translation.
Mouse Monoclonal Anti-Zaire-Ebola virus (killed) IgG #2, aff pure
EVZ13-M
Alpha Diagnostics
100 ul
EUR 578.4
Mouse Monoclonal Anti-Sudan Ebola virus glycoprotein (SUDV GP) IgG, purified
SVGP22-M
Alpha Diagnostics
100 ul
EUR 578.4
Ebola Virus VP40 protein
30-1837
Fitzgerald
100 ug
EUR 211.2
Description: Recombinant Ebola Virus VP40 Matrix protein
Ebola Virus VP40 antibody
10-2351
Fitzgerald
250 ug
EUR 457.2
Description: Mouse Monoclonal Ebola Virus VP40 antibody
Ebola Virus VP40 antibody
10-2352
Fitzgerald
250 ug
EUR 457.2
Description: Mouse Monoclonal Ebola Virus VP40 antibody
Ebola virus Nucleoprotein Antibody
abx023955-1mg
Abbexa
1 mg
EUR 1212
Ebola virus Nucleoprotein Antibody
abx023956-1mg
Abbexa
1 mg
EUR 1212
Ebola virus Nucleoprotein Antibody
abx023957-1mg
Abbexa
1 mg
EUR 1212
Ebola virus Nucleoprotein Antibody
abx023958-1mg
Abbexa
1 mg
EUR 1212
Ebola Virus NP protein
80-1411
Fitzgerald
100 ug
EUR 211.2
Description: Purified Recombinant Ebola Nucleoprotein antigen (Zaire strain)
Mouse antibody for Ebola virus NP
2601
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus NP for WB, ELISA.
Mouse antibody for Ebola virus NP
2603
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus NP for WB, ELISA.
Mouse antibody for Ebola virus NP
2604
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus NP for WB, ELISA.
Mouse antibody for Ebola virus VP40
2611
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.
Mouse antibody for Ebola virus VP40
2612
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.
Mouse antibody for Ebola virus VP40
2613
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.
Mouse antibody for Ebola virus VP40
2616
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.
Recombinant Ebola virus Ebola Virus-like particles Protein, Native, Human-100ug
QP10485-100ug
EnQuireBio
100ug
EUR 925.2
Monoclonal Anti-Zaire-Ebola virus VP40 (ZEBOV VP40) IgG, aff pure
EVP407-M
Alpha Diagnostics
100 ul
EUR 578.4
Monoclonal Anti-Zaire-Ebola virus VP40 (ZEBOV VP40) IgG, aff pure
EVP408-M
Alpha Diagnostics
100 ul
EUR 578.4
Ebola virus NP recombinant antigen
8828
Virostat
100 ug
EUR 673.36
Description: This is Ebola virus NP recombinant antigen for ELISA,WB.
Human Monoclonal Anti-Falvivirus (Ebola and Marburg virus glycoprotein, GP) IgG (Neutralzing)
EVGP26-M
Alpha Diagnostics
100 ul
EUR 578.4
Recombinant Ebola Virus Ebola Sudan Protein Protein, Untagged, E.coli-100ug
QP11726-100ug
EnQuireBio
100ug
EUR 261.6
Recombinant Ebola Virus Ebola Sudan Protein Protein, Untagged, E.coli-1mg
QP11726-1mg
EnQuireBio
1mg
EUR 1513.2
Recombinant Ebola Virus Ebola Sudan Protein Protein, Untagged, E.coli-500ug
QP11726-500ug
EnQuireBio
500ug
EUR 795.6
Recombinant Ebola Virus Ebola Zaire GP Protein, His, E.coli-100ug
QP11727-100ug
EnQuireBio
100ug
EUR 261.6
Recombinant Ebola Virus Ebola Zaire GP Protein, His, E.coli-1mg
QP11727-1mg
EnQuireBio
1mg
EUR 1513.2
Recombinant Ebola Virus Ebola Zaire GP Protein, His, E.coli-500ug
QP11727-500ug
EnQuireBio
500ug
EUR 795.6
Recombinant Ebola Virus Ebola Zaire Protein Protein, His, E.coli-100ug
QP11728-100ug
EnQuireBio
100ug
EUR 261.6
Recombinant Ebola Virus Ebola Zaire Protein Protein, His, E.coli-1mg
QP11728-1mg
EnQuireBio
1mg
EUR 1513.2
Recombinant Ebola Virus Ebola Zaire Protein Protein, His, E.coli-500ug
QP11728-500ug
EnQuireBio
500ug
EUR 795.6
Recombinant Ebola Virus Ebola Zaire VP40 Protein, His, E.coli-100ug
QP11729-100ug
EnQuireBio
100ug
EUR 261.6
Recombinant Ebola Virus Ebola Zaire VP40 Protein, His, E.coli-1mg
QP11729-1mg
EnQuireBio
1mg
EUR 1513.2
Recombinant Ebola Virus Ebola Zaire VP40 Protein, His, E.coli-500ug
QP11729-500ug
EnQuireBio
500ug
EUR 795.6
Ebola virus-like particles (EBOV VLP)
0550-001
IBT Bioservices
1mg
EUR 662.4
Description:
Related to: FilovirusesApplications: WB
Rabbit Anti-Sudan Ebola virus Nucleoprotein (SUDV NP) IgG
SVNP25-A
Alpha Diagnostics
100 ul
EUR 578.4
Mouse Monoclonal Anti-Rabies Virus antiserum
RBV13-S
Alpha Diagnostics
100 ul
EUR 548.4
Rabbit Polyclonal antibody Anti-CRBN
Anti-CRBN
ImmunoStep
50 µg
EUR 418.8
Mouse Anti-Zaire Ebola virus VP40 IgG ELISA Kit, 96 tests, Quantitative
AE-320700-1
Alpha Diagnostics
1 kit
EUR 1074
Mouse Anti-Zaire Ebola virus VP40 IgM ELISA Kit, 96 tests, Quantitative
AE-320710-1
Alpha Diagnostics
1 kit
EUR 1074
Monoclonal Anti-Mumps virus (Enders) Virus IgG
MUMS12-M
Alpha Diagnostics
100 ul
EUR 489.6
Ebola virus Viral Protein 40 (VP40) Antibody
abx023959-1mg
Abbexa
1 mg
EUR 1212
Ebola virus Viral Protein 40 (VP40) Antibody
abx023960-1mg
Abbexa
1 mg
EUR 1212
Ebola virus Viral Protein 40 (VP40) Antibody
abx023961-1mg
Abbexa
1 mg
EUR 1212
Ebola virus Viral Protein 40 (VP40) Antibody
abx023962-1mg
Abbexa
1 mg
EUR 1212
Ebola virus Viral Protein 40 (VP40) Antibody
abx023963-1mg
Abbexa
1 mg
EUR 1212
Ebola virus Viral Protein 40 (VP40) Antibody
abx023964-1mg
Abbexa
1 mg
EUR 1212
Mouse Monoclonal Anti-Zaire Ebola virus (killed) IgG (mixture of EVZ12-M and EVZ13-M), aff pure
EVZ14-M
Alpha Diagnostics
100 ul
EUR 578.4
Mouse Anti-Canine Distemper Virus monoclonal antibody
CABT-BL8819
Creative Diagnostics
1.0 mg
EUR 1070.4
Inhibition of PIKfyve kinase prevents an infection by Zaire ebolavirus and SARS-CoV-2 Virus entry is a multistep course of. It initiates when the virus attaches to the host cell and ends when the viral contents attain the cytosol. Genetically unrelated viruses can subvert analogous subcellular mechanisms and use comparable trafficking pathways for profitable entry.
Antiviral methods concentrating on early steps of an infection are subsequently interesting, notably when the likelihood for profitable interference via a typical step is highest. We describe right here potent inhibitory results on content material launch and an infection by chimeric vesicular stomatitis virus (VSV ) containing the envelope proteins of Zaire ebolavirus (VSV-ZEBOV) or extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (VSV-SARS-CoV-2) elicited by Apilimod and Vacuolin-1, small-molecule inhibitors of the primary endosomal phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, PIKfyve.
We additionally describe potent inhibition of SARS-CoV-2 pressure 2019-nCoV/USA-WA1/2020 by Apilimod. These outcomes outline instruments for learning the intracellular trafficking of pathogens elicited by inhibition of PIKfyve kinase and recommend the potential for concentrating on this kinase in growing small-molecule antivirals towards SARS-CoV-2.
Key phrases: APILIMOD; COVID-19; SARS-CoV-2; Vacuolin-1; ZEBOV
Rabbit anti-EBOV VLP antiserum
01-0004
IBT Bioservices
250uL
EUR 460.8
Description:
Related to: FilovirusesApplications: ELISA, WB
Rabbit anti-EBOV VLP pAb
0315-001
IBT Bioservices
500ug
EUR 577.2
Description:
Related to: FilovirusesApplications: ELISA, WB
Ebola Virus antibody
10-2253
Fitzgerald
250 ug
EUR 363.6
Description: Mouse monoclonal Ebola Virus antibody
Ebola Virus antibody
10-2254
Fitzgerald
250 ug
EUR 354
Description: Mouse monoclonal Ebola Virus antibody
Ebola Virus antibody
10-2255
Fitzgerald
250 ug
EUR 418.8
Description: Mouse monoclonal Ebola Virus antibody
Ebola Virus Antibody
abx021693-1mg
Abbexa
1 mg
EUR 1279.2
Ebola Virus Antibody
abx021694-1mg
Abbexa
1 mg
EUR 1279.2
Ebola Virus Antibody
abx023559-1mg
Abbexa
1 mg
EUR 1195.2
Rabbit Anti-Zaire-Ebola virus nucleoprotein (Mayinga EBOV NP) protein antiserum
EVNP11-S
Alpha Diagnostics
100 ul
EUR 548.4
Zaire Ebola Virus Glycoprotein (EBOV GP antigen) ELISA Kit, 48 tests, Quantitative
AE-320800-48
Alpha Diagnostics
1 kit
EUR 1074
Zaire Ebola Virus Glycoprotein (EBOV GP antigen) ELISA Kit, 96 tests, Quantitative
AE-320800-96
Alpha Diagnostics
1 kit
EUR 1804.8
Mouse Monoclonal Anti-Anti-Zaire Ebola virus glycoprotein (EBOV GP) IgG, purified
EVGP20-M
Alpha Diagnostics
100 ul
EUR 578.4
Recombinant Zaire-Ebola virus nucleoprotein (Mayinga EBOV NP) protein control for Western
EVNP11-C
Alpha Diagnostics
100 ul
EUR 343.2
Ebola Virus VP40 protein
30-1837
Fitzgerald
100 ug
EUR 211.2
Description: Recombinant Ebola Virus VP40 Matrix protein
Ebola Virus VP40 antibody
10-2351
Fitzgerald
250 ug
EUR 457.2
Description: Mouse Monoclonal Ebola Virus VP40 antibody
Ebola Virus VP40 antibody
10-2352
Fitzgerald
250 ug
EUR 457.2
Description: Mouse Monoclonal Ebola Virus VP40 antibody
Ebola virus Nucleoprotein Antibody
abx023955-1mg
Abbexa
1 mg
EUR 1212
Ebola virus Nucleoprotein Antibody
abx023956-1mg
Abbexa
1 mg
EUR 1212
Ebola virus Nucleoprotein Antibody
abx023957-1mg
Abbexa
1 mg
EUR 1212
Ebola virus Nucleoprotein Antibody
abx023958-1mg
Abbexa
1 mg
EUR 1212
Ebola Virus NP protein
80-1411
Fitzgerald
100 ug
EUR 211.2
Description: Purified Recombinant Ebola Nucleoprotein antigen (Zaire strain)
Rabbit Anti-Zaire Ebola virus nucleoprotein (EBOV NP, 1-739/DNA vaccine) IgG
EVNP13-A
Alpha Diagnostics
100 ul
EUR 578.4
Recombinant (E. coli) Zaire-Ebola virus nucleoprotein (Mayinga EBOV NP) protein control for Western
EVNP13-C
Alpha Diagnostics
100 ul
EUR 343.2
Rabbit Anti-Zaire-Ebola virus nucleoprotein (Gulu, EBOV NP/630-738aa) IgG, aff pure
EVNP17-A
Alpha Diagnostics
100 ul
EUR 578.4
Ebola virus NP recombinant antigen
8828
Virostat
100 ug
EUR 673.36
Description: This is Ebola virus NP recombinant antigen for ELISA,WB.
Recombinant (E.coli) Zaire Ebola virus nucleoprotein (EBOV NP) (full length, his-tag, 82 kda), purified
EVNP15-R-10
Alpha Diagnostics
10 ug
EUR 416.4
Recombinant (E.coli) Zaire Ebola virus nucleoprotein (EBOV NP) (full length, his-tag, 82 kda), purified
EVNP15-R-100
Alpha Diagnostics
100 ug
EUR 2617.2
Recombinant Ebola Virus Ebola Sudan Protein Protein, Untagged, E.coli-100ug
QP11726-100ug
EnQuireBio
100ug
EUR 261.6
Recombinant Ebola Virus Ebola Sudan Protein Protein, Untagged, E.coli-1mg
QP11726-1mg
EnQuireBio
1mg
EUR 1513.2
Recombinant Ebola Virus Ebola Sudan Protein Protein, Untagged, E.coli-500ug
QP11726-500ug
EnQuireBio
500ug
EUR 795.6
Recombinant Ebola Virus Ebola Zaire GP Protein, His, E.coli-100ug
QP11727-100ug
EnQuireBio
100ug
EUR 261.6
Recombinant Ebola Virus Ebola Zaire GP Protein, His, E.coli-1mg
QP11727-1mg
EnQuireBio
1mg
EUR 1513.2
Recombinant Ebola Virus Ebola Zaire GP Protein, His, E.coli-500ug
QP11727-500ug
EnQuireBio
500ug
EUR 795.6
Recombinant Ebola Virus Ebola Zaire Protein Protein, His, E.coli-100ug
QP11728-100ug
EnQuireBio
100ug
EUR 261.6
Recombinant Ebola Virus Ebola Zaire Protein Protein, His, E.coli-1mg
QP11728-1mg
EnQuireBio
1mg
EUR 1513.2
Recombinant Ebola Virus Ebola Zaire Protein Protein, His, E.coli-500ug
QP11728-500ug
EnQuireBio
500ug
EUR 795.6
Recombinant Ebola Virus Ebola Zaire VP40 Protein, His, E.coli-100ug
QP11729-100ug
EnQuireBio
100ug
EUR 261.6
Recombinant Ebola Virus Ebola Zaire VP40 Protein, His, E.coli-1mg
QP11729-1mg
EnQuireBio
1mg
EUR 1513.2
Recombinant Ebola Virus Ebola Zaire VP40 Protein, His, E.coli-500ug
QP11729-500ug
EnQuireBio
500ug
EUR 795.6
Mouse antibody for Ebola virus NP
2601
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus NP for WB, ELISA.
Mouse antibody for Ebola virus NP
2603
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus NP for WB, ELISA.
Mouse antibody for Ebola virus NP
2604
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus NP for WB, ELISA.
Mouse antibody for Ebola virus VP40
2611
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.
Mouse antibody for Ebola virus VP40
2612
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.
Mouse antibody for Ebola virus VP40
2613
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.
Mouse antibody for Ebola virus VP40
2616
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.
Recombinant (E.coli) Zaire Ebola virus nucleoprotein (EBOV NP) (full length, his-tag, 82 kda)-Biotin Conjugate
EVNP15-BTN
Alpha Diagnostics
100 ul
EUR 489.6
Recombinant Chikungunya Virus VLP Protein, Native, Human-100ug
QP10473-100ug
EnQuireBio
100ug
EUR 882
Recombinant Chikungunya Virus VLP Protein, Native, Human-500ug
QP10473-500ug
EnQuireBio
500ug
EUR 3145.2
Mouse anti-Ebola Virus Nucleoprotein monoclonal antibody
DMAB-A005
Creative Diagnostics
1 mg
EUR 1552.8
Mouse anti-Ebola Virus Nucleoprotein monoclonal antibody
DMAB-A006
Creative Diagnostics
1 mg
EUR 1552.8
Ebola virus Viral Protein 40 (VP40) Antibody
abx023959-1mg
Abbexa
1 mg
EUR 1212
Ebola virus Viral Protein 40 (VP40) Antibody
abx023960-1mg
Abbexa
1 mg
EUR 1212
Ebola virus Viral Protein 40 (VP40) Antibody
abx023961-1mg
Abbexa
1 mg
EUR 1212
Ebola virus Viral Protein 40 (VP40) Antibody
abx023962-1mg
Abbexa
1 mg
EUR 1212
Ebola virus Viral Protein 40 (VP40) Antibody
abx023963-1mg
Abbexa
1 mg
EUR 1212
Ebola virus Viral Protein 40 (VP40) Antibody
abx023964-1mg
Abbexa
1 mg
EUR 1212
Recombinant Dengue virus Dengue Virus Serotype 4 VLP Protein, Native, Human-100ug
QP10484-100ug
EnQuireBio
100ug
EUR 1099.2
Recombinant Dengue virus Dengue Virus Serotype 4 VLP Protein, Native, Human-250ug
QP10484-250ug
EnQuireBio
250ug
EUR 2144.4
Recombinant Human Papilloma Virus type 6 L1 protein (VLP)
DAGF-227
Creative Diagnostics
1mg
EUR 1189.2
Recombinant Human Papilloma Virus type 11 L1 protein (VLP)
DAGF-228
Creative Diagnostics
1mg
EUR 1189.2
Recombinant Human Papilloma Virus type 16 L1 protein (VLP)
DAGF-229
Creative Diagnostics
1mg
EUR 1189.2
Recombinant Human Papilloma Virus type 18 L1 protein (VLP)
DAGF-230
Creative Diagnostics
1mg
EUR 1189.2
Recombinant Human Papilloma Virus type 31 L1 protein (VLP)
DAGF-231
Creative Diagnostics
1mg
EUR 1189.2
Recombinant Human Papilloma Virus type 33 L1 protein (VLP)
DAGF-232
Creative Diagnostics
1mg
EUR 1189.2
Recombinant Human Papilloma Virus type 45 L1 protein (VLP)
DAGF-233
Creative Diagnostics
1mg
EUR 1189.2
Recombinant Human Papilloma Virus type 52 L1 protein (VLP)
DAGF-234
Creative Diagnostics
1mg
EUR 1189.2
Recombinant (E.coli) Zaire Ebola virus nucleoprotein (EBOV NP) (H.sapiens-wt/GIN/2014/Kissidougou-C15, 630-739aa, his-tag, >95%)
EVNP16-R-10
Alpha Diagnostics
10 ug
EUR 416.4
Recombinant (E.coli) Zaire Ebola virus nucleoprotein (EBOV NP) (H.sapiens-wt/GIN/2014/Kissidougou-C15, 630-739aa , his-tag, >95%)
EVNP16-R-100
Alpha Diagnostics
100 ug
EUR 2617.2
Ebola hemorrhagic fever Virus (Sudan, SEBOV) PCR kit
PCR-H703-48R
Bioingentech
50T
EUR 987.6
Ebola hemorrhagic fever Virus (Sudan, SEBOV) PCR kit
PCR-H703-96R
Bioingentech
100T
EUR 1335.6
Recombinant Dengue Virus Serotype 1 VLP Protein, Native, Human-100ug
QP10476-100ug
EnQuireBio
100ug
EUR 1099.2
Recombinant Dengue Virus Serotype 1 VLP Protein, Native, Human-250ug
QP10476-250ug
EnQuireBio
250ug
EUR 2350.8
Recombinant Dengue Virus Serotype 1 VLP Protein, Native, Human-500ug
QP10476-500ug
EnQuireBio
500ug
EUR 3548.4
