The Minor Matrix Protein VP24 from Ebola Virus Lacks Direct Lipid-Binding Properties. Viral protein 24 (VP24) from Ebola virus (EBOV) was first acknowledged as a minor matrix protein that associates with mobile membranes. Nonetheless, more moderen research make clear its roles in inhibiting viral genome transcription and replication, facilitating nucleocapsid meeting and transport, and interfering with immune responses in host cells via downregulation of interferon (IFN)-activated genes.
Thus, whether or not VP24 is a peripheral protein with lipid-binding potential for matrix layer recruitment has not been explored. Right here, we examined the lipid-binding potential of VP24 with quite a lot of lipid-binding assays. The outcomes indicated that VP24 lacked the power to affiliate with lipids examined no matter VP24 posttranslational modifications.
We additional display that the presence of the EBOV main matrix protein VP40 didn’t promote VP24 membrane affiliation in vitro or in cells. Additional, no protein-protein interactions between VP24 and VP40 had been detected by co-immunoprecipitation. Confocal imaging and mobile membrane fractionation analyses in human cells advised VP24 didn’t particularly localize on the plasma membrane internal leaflet.
General, we offer proof that EBOV VP24 will not be a lipid-binding protein and its presence within the viral matrix layer is probably going not depending on direct lipid interactions.
Key phrases: Ebola virus; VP24; filovirus; lipid binding; matrix protein.
Anticancer and Antiviral Properties of Cardiac Glycosides: A Overview to Discover the Mechanism of Actions Cardiac glycosides (CGs) have an extended historical past of treating cardiac ailments. Nonetheless, current experiences have advised that CGs additionally possess anticancer and antiviral actions. The first mechanism of motion of those anticancer brokers is by suppressing the Na+ /ok+ -ATPase by reducing the intracellular Okay+ and growing the Na+ and Ca2+ . Moreover, CGs had been recognized to behave as inhibitors of IL8 manufacturing, DNA topoisomerase I and II, anoikis prevention and suppression of a number of goal genes chargeable for the inhibition of most cancers cell proliferation.
Furthermore, CGs had been reported to be efficient towards a number of DNA and RNA viral species equivalent to influenza, human cytomegalovirus, herpes simplex virus, coronavirus, tick-borne encephalitis (TBE) virus and Ebola virus. CGs had been reported to suppress the HIV-1 gene expression, viral protein translation and alters viral pre-mRNA splicing to inhibit the viral replication.
Thus far, 4 CGs (Anvirzel, UNBS1450, PBI05204 and digoxin) had been in medical trials for his or her anticancer exercise . This evaluate encapsulates the present data about CGs as anticancer and antiviral medication in isolation and together with another medication to boost their effectivity. Additional research of this class of biomolecules are mandatory to find out their attainable inhibitory position in most cancers and viral ailments.
zebov-detection
Key phrases: anticancer; antiviral; autophagy; biomolecules; cardiac glycosides; preclinical trials; signaling pathway; viral protein translation.
Mouse antibody for Ebola virus NP
2603
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus NP for WB, ELISA.
Mouse antibody for Ebola virus NP
2604
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus NP for WB, ELISA.
Mouse antibody for Ebola virus VP40
2611
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.
Mouse antibody for Ebola virus VP40
2612
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.
Mouse antibody for Ebola virus VP40
2613
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.
Mouse antibody for Ebola virus VP40
2614
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.
Mouse antibody for Ebola virus VP40
2615
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.
Mouse antibody for Ebola virus VP40
2616
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.
Mouse Monoclonal Anti-human Ebola receptor (KIM1/TIM1) (21-290 aa) IgG, aff pure
TIM13-M
Alpha Diagnostics
100 ul
EUR 578.4
Ebola Virus antibody
10-2253
Fitzgerald
250 ug
EUR 363.6
Description: Mouse monoclonal Ebola Virus antibody
Ebola Virus antibody
10-2254
Fitzgerald
250 ug
EUR 354
Description: Mouse monoclonal Ebola Virus antibody
Ebola Virus antibody
10-2255
Fitzgerald
250 ug
EUR 418.8
Description: Mouse monoclonal Ebola Virus antibody
Ebola Virus Antibody
abx021693-1mg
Abbexa
1 mg
EUR 1279.2
Ebola Virus Antibody
abx021694-1mg
Abbexa
1 mg
EUR 1279.2
Ebola Virus Antibody
abx023559-1mg
Abbexa
1 mg
EUR 1195.2
Mouse Monoclonal Anti-Rabies Virus antiserum
RBV13-S
Alpha Diagnostics
100 ul
EUR 548.4
Mouse Anti Marburg Virus 30Kda Monoclonal Antibody
DMABT-51433MM
Creative Diagnostics
0.1 mg
EUR 1132.8
Mouse Anti Marek's Disease Virus Monoclonal Antibody
DMABT-51435MM
Creative Diagnostics
0.2 mg
EUR 889.2
Mouse Monoclonal (3576) Anti-Yellow Fever Virus IgG2a
YFV11-M
Alpha Diagnostics
100 ul
EUR 578.4
Mouse Anti Herpes Simplex Virus Monoclonal Antibody
DMABT-51352MH
Creative Diagnostics
0.1 mg
EUR 1008
Mouse Anti-Marek Disease Virus Monoclonal Antibody
DMAB3903-05mg
Creative Diagnostics
0.5 mg
EUR 1076.4
Mouse Anti-Marek Disease Virus Monoclonal Antibody
DMAB3903-1mg
Creative Diagnostics
1 mg
EUR 1606.8
Mouse Anti-Marek Disease Virus Monoclonal Antibody
DMAB3906-05mg
Creative Diagnostics
0.5 mg
EUR 1076.4
Mouse Anti-Marek Disease Virus Monoclonal Antibody
DMAB3906-1mg
Creative Diagnostics
1 mg
EUR 1606.8
Mouse Anti-Marek Disease Virus Monoclonal Antibody
DMAB3907-05mg
Creative Diagnostics
0.5 mg
EUR 1076.4
Mouse Anti-Marek Disease Virus Monoclonal Antibody
DMAB3907-1mg
Creative Diagnostics
1 mg
EUR 1606.8
Mouse Anti Parainfluenza Virus 1 Monoclonal Antibody
DMABT-51474MP
Creative Diagnostics
0.1 mg
EUR 1070.4
Mouse Anti Parainfluenza Virus 1 Monoclonal Antibody
DMABT-51475MP
Creative Diagnostics
0.1 mg
EUR 1070.4
Mouse Anti Parainfluenza Virus 1 Monoclonal Antibody
DMABT-51476MP
Creative Diagnostics
0.1 mg
EUR 1070.4
Mouse Anti Parainfluenza Virus 1 Monoclonal Antibody
DMABT-51477MP
Creative Diagnostics
1 mg
EUR 1057.2
Mouse Anti Parainfluenza Virus 1 Monoclonal Antibody
DMABT-51478MP
Creative Diagnostics
0.1 mg
EUR 1070.4
Mouse Anti Parainfluenza Virus 1 Monoclonal Antibody
DMABT-51479MP
Creative Diagnostics
0.1 mg
EUR 1070.4
Mouse Anti Parainfluenza Virus 2 Monoclonal Antibody
DMABT-51480MP
Creative Diagnostics
0.1 mg
EUR 1070.4
Mouse Anti Parainfluenza Virus 2 Monoclonal Antibody
DMABT-51481MP
Creative Diagnostics
0.1 mg
EUR 1070.4
Mouse Anti Parainfluenza Virus 3 Monoclonal Antibody
DMABT-51482MP
Creative Diagnostics
0.1 mg
EUR 1070.4
Mouse Anti Parainfluenza Virus 3 Monoclonal Antibody
DMABT-51483MP
Creative Diagnostics
0.1 mg
EUR 1070.4
Mouse Anti Parainfluenza Virus 3 Monoclonal Antibody
DMABT-51484MP
Creative Diagnostics
1 mg
EUR 1057.2
Mouse Anti Dengue Virus Type 2 Monoclonal Antibody
DMABT-51213MD
Creative Diagnostics
0.1 mg
EUR 1057.2
Mouse Anti Dengue Virus Type 2 Monoclonal Antibody
DMABT-51214MD
Creative Diagnostics
0.5 mg
EUR 1057.2
Mouse Anti Newcastle Disease Virus Monoclonal Antibody
DMABT-51460MN
Creative Diagnostics
0.2 mg
EUR 920.4
Mouse Anti-Canine Distemper Virus monoclonal antibody
CABT-BL8819
Creative Diagnostics
1.0 mg
EUR 1070.4
Mouse Anti-Canine Distemper Virus monoclonal antibody
CABT-BL8820
Creative Diagnostics
1.0 mg
EUR 1070.4
Mouse Anti-Newcastle Disease Virus Monoclonal Antibody
DMAB4005-05mg
Creative Diagnostics
0.5 mg
EUR 1076.4
Mouse Anti-Newcastle Disease Virus Monoclonal Antibody
DMAB4005-1mg
Creative Diagnostics
1 mg
EUR 1606.8
Mouse Anti-Newcastle Disease Virus Monoclonal Antibody
DMAB4006-05mg
Creative Diagnostics
0.5 mg
EUR 1076.4
Mouse Anti-Newcastle Disease Virus Monoclonal Antibody
DMAB4006-1mg
Creative Diagnostics
1 mg
EUR 1606.8
Mouse Anti-Newcastle Disease Virus Monoclonal Antibody
DMAB4009-05mg
Creative Diagnostics
0.5 mg
EUR 1076.4
Mouse Anti-Newcastle Disease Virus Monoclonal Antibody
DMAB4009-1mg
Creative Diagnostics
1 mg
EUR 1606.8
Mouse Anti-Newcastle Disease Virus Monoclonal Antibody
DMAB4010-05mg
Creative Diagnostics
0.5 mg
EUR 1076.4
Inhibition of PIKfyve kinase prevents an infection by Zaire ebolavirus and SARS-CoV-2 Virus entry is a multistep course of. It initiates when the virus attaches to the host cell and ends when the viral contents attain the cytosol. Genetically unrelated viruses can subvert analogous subcellular mechanisms and use comparable trafficking pathways for profitable entry.
Antiviral methods concentrating on early steps of an infection are subsequently interesting, notably when the likelihood for profitable interference via a typical step is highest. We describe right here potent inhibitory results on content material launch and an infection by chimeric vesicular stomatitis virus (VSV ) containing the envelope proteins of Zaire ebolavirus (VSV-ZEBOV) or extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (VSV-SARS-CoV-2) elicited by Apilimod and Vacuolin-1, small-molecule inhibitors of the primary endosomal phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, PIKfyve.
We additionally describe potent inhibition of SARS-CoV-2 pressure 2019-nCoV/USA-WA1/2020 by Apilimod. These outcomes outline instruments for learning the intracellular trafficking of pathogens elicited by inhibition of PIKfyve kinase and recommend the potential for concentrating on this kinase in growing small-molecule antivirals towards SARS-CoV-2.
Key phrases: APILIMOD; COVID-19; SARS-CoV-2; Vacuolin-1; ZEBOV
Rabbit Anti-Zaire Ebola virus nucleoprotein (EBOV NP, 1-739/DNA vaccine) IgG
EVNP13-A
Alpha Diagnostics
100 ul
EUR 578.4
Zaire Ebola Virus Glycoprotein (EBOV GP antigen) ELISA Kit, 48 tests, Quantitative
AE-320800-48
Alpha Diagnostics
1 kit
EUR 1074
Zaire Ebola Virus Glycoprotein (EBOV GP antigen) ELISA Kit, 96 tests, Quantitative
AE-320800-96
Alpha Diagnostics
1 kit
EUR 1804.8
Mouse Monoclonal Anti-Anti-Zaire Ebola virus glycoprotein (EBOV GP) IgG, purified
EVGP20-M
Alpha Diagnostics
100 ul
EUR 578.4
Ebola Virus antibody
10-2253
Fitzgerald
250 ug
EUR 363.6
Description: Mouse monoclonal Ebola Virus antibody
Ebola Virus antibody
10-2254
Fitzgerald
250 ug
EUR 354
Description: Mouse monoclonal Ebola Virus antibody
Ebola Virus antibody
10-2255
Fitzgerald
250 ug
EUR 418.8
Description: Mouse monoclonal Ebola Virus antibody
Ebola Virus Antibody
abx021693-1mg
Abbexa
1 mg
EUR 1279.2
Ebola Virus Antibody
abx021694-1mg
Abbexa
1 mg
EUR 1279.2
Ebola Virus Antibody
abx023559-1mg
Abbexa
1 mg
EUR 1195.2
Ebola Virus NP protein
80-1411
Fitzgerald
100 ug
EUR 211.2
Description: Purified Recombinant Ebola Nucleoprotein antigen (Zaire strain)
Ebola Virus VP40 protein
30-1837
Fitzgerald
100 ug
EUR 211.2
Description: Recombinant Ebola Virus VP40 Matrix protein
Ebola Virus VP40 antibody
10-2351
Fitzgerald
250 ug
EUR 457.2
Description: Mouse Monoclonal Ebola Virus VP40 antibody
Ebola Virus VP40 antibody
10-2352
Fitzgerald
250 ug
EUR 457.2
Description: Mouse Monoclonal Ebola Virus VP40 antibody
Recombinant (E.coli) Zaire Ebola virus nucleoprotein (EBOV NP) (full length, his-tag, 82 kda), purified
EVNP15-R-10
Alpha Diagnostics
10 ug
EUR 416.4
Recombinant (E.coli) Zaire Ebola virus nucleoprotein (EBOV NP) (full length, his-tag, 82 kda), purified
EVNP15-R-100
Alpha Diagnostics
100 ug
EUR 2617.2
Ebola virus Nucleoprotein Antibody
abx023955-1mg
Abbexa
1 mg
EUR 1212
Ebola virus Nucleoprotein Antibody
abx023956-1mg
Abbexa
1 mg
EUR 1212
Ebola virus Nucleoprotein Antibody
abx023957-1mg
Abbexa
1 mg
EUR 1212
Ebola virus Nucleoprotein Antibody
abx023958-1mg
Abbexa
1 mg
EUR 1212
Recombinant (E.coli) Zaire Ebola virus nucleoprotein (EBOV NP) (full length, his-tag, 82 kda)-Biotin Conjugate
EVNP15-BTN
Alpha Diagnostics
100 ul
EUR 489.6
Ebola virus NP recombinant antigen
8828
Virostat
100 ug
EUR 673.36
Description: This is Ebola virus NP recombinant antigen for ELISA,WB.
Recombinant Ebola Virus Ebola Zaire GP Protein, His, E.coli-1mg
QP11727-1mg
EnQuireBio
1mg
EUR 1513.2
Mouse antibody for Ebola virus NP
2601
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus NP for WB, ELISA.
Mouse antibody for Ebola virus NP
2602
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus NP for WB, ELISA.
Mouse antibody for Ebola virus NP
2603
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus NP for WB, ELISA.
Mouse antibody for Ebola virus NP
2604
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus NP for WB, ELISA.
Recombinant Ebola Virus Ebola Zaire GP Protein, His, E.coli-100ug
QP11727-100ug
EnQuireBio
100ug
EUR 261.6
Recombinant Ebola Virus Ebola Zaire GP Protein, His, E.coli-500ug
QP11727-500ug
EnQuireBio
500ug
EUR 795.6
Recombinant Ebola Virus Ebola Zaire VP40 Protein, His, E.coli-1mg
QP11729-1mg
EnQuireBio
1mg
EUR 1513.2
Recombinant Ebola Virus Ebola Zaire VP40 Protein, His, E.coli-100ug
QP11729-100ug
EnQuireBio
100ug
EUR 261.6
Recombinant Ebola Virus Ebola Zaire VP40 Protein, His, E.coli-500ug
QP11729-500ug
EnQuireBio
500ug
EUR 795.6
Mouse antibody for Ebola virus VP40
2611
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.
Mouse antibody for Ebola virus VP40
2612
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.
Mouse antibody for Ebola virus VP40
2613
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.
Mouse antibody for Ebola virus VP40
2614
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.
Mouse antibody for Ebola virus VP40
2615
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.
Mouse antibody for Ebola virus VP40
2616
Virostat
100 ug
EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.
Recombinant Ebola Virus Ebola Zaire Protein Protein, His, E.coli-1mg
QP11728-1mg
EnQuireBio
1mg
EUR 1513.2
Recombinant Ebola Virus Ebola Zaire Protein Protein, His, E.coli-100ug
QP11728-100ug
EnQuireBio
100ug
EUR 261.6
Recombinant Ebola Virus Ebola Zaire Protein Protein, His, E.coli-500ug
QP11728-500ug
EnQuireBio
500ug
EUR 795.6
Recombinant Ebola Virus Ebola Sudan Protein Protein, Untagged, E.coli-1mg
QP11726-1mg
EnQuireBio
1mg
EUR 1513.2
Recombinant Ebola Virus Ebola Sudan Protein Protein, Untagged, E.coli-100ug
QP11726-100ug
EnQuireBio
100ug
EUR 261.6
Recombinant Ebola Virus Ebola Sudan Protein Protein, Untagged, E.coli-500ug
QP11726-500ug
EnQuireBio
500ug
EUR 795.6
Recombinant (E.coli) Zaire Ebola virus nucleoprotein (EBOV NP) (H.sapiens-wt/GIN/2014/Kissidougou-C15, 630-739aa, his-tag, >95%)
EVNP16-R-10
Alpha Diagnostics
10 ug
EUR 416.4
Recombinant (E.coli) Zaire Ebola virus nucleoprotein (EBOV NP) (H.sapiens-wt/GIN/2014/Kissidougou-C15, 630-739aa , his-tag, >95%)
EVNP16-R-100
Alpha Diagnostics
100 ug
EUR 2617.2
Ebola virus Viral Protein 40 (VP40) Antibody
abx023959-1mg
Abbexa
1 mg
EUR 1212
Ebola virus Viral Protein 40 (VP40) Antibody
abx023960-1mg
Abbexa
1 mg
EUR 1212
Ebola virus Viral Protein 40 (VP40) Antibody
abx023961-1mg
Abbexa
1 mg
EUR 1212
Ebola virus Viral Protein 40 (VP40) Antibody
abx023962-1mg
Abbexa
1 mg
EUR 1212
Ebola virus Viral Protein 40 (VP40) Antibody
abx023963-1mg
Abbexa
1 mg
EUR 1212
Ebola virus Viral Protein 40 (VP40) Antibody
abx023964-1mg
Abbexa
1 mg
EUR 1212
Rabbit Anti-Sudan Ebola virus Nucleoprotein (SUDV NP) IgG
SVNP25-A
Alpha Diagnostics
100 ul
EUR 578.4
Ebola hemorrhagic fever Virus (Sudan, SEBOV) PCR kit
PCR-H703-48R
Bioingentech
50T
EUR 987.6
Ebola hemorrhagic fever Virus (Sudan, SEBOV) PCR kit
PCR-H703-96R
Bioingentech
100T
EUR 1335.6
Ebola hemorrhagic fever Virus (Sudan, SEBOV) RT PCR kit
RTq-H703-100R
Bioingentech
100T
EUR 1573.2
Ebola hemorrhagic fever Virus (Sudan, SEBOV) RT PCR kit
RTq-H703-150R
Bioingentech
150T
EUR 2144.4
Ebola hemorrhagic fever Virus (Sudan, SEBOV) RT PCR kit
RTq-H703-50R
Bioingentech
50T
EUR 1155.6
Rabbit Anti-Zaire-Ebola virus nucleoprotein (Gulu, EBOV NP/630-738aa) IgG, aff pure
EVNP17-A
Alpha Diagnostics
100 ul
EUR 578.4
Recombinant (E.coli) Sudan Ebola virus VP40 (Uganda,31-326aa, His-tag, >95%)
SVP407-R-10
Alpha Diagnostics
10 ug
EUR 416.4
Recombinant (E.coli) Sudan Ebola virus VP40 (Uganda,31-326aa, His-tag, >95%)
SVP407-R-100
Alpha Diagnostics
100 ug
EUR 2617.2
Mouse Anti-Zaire Ebola virus VP40 IgG ELISA Kit, 96 tests, Quantitative
AE-320700-1
Alpha Diagnostics
1 kit
EUR 1074
Mouse Anti-Zaire Ebola virus VP40 IgM ELISA Kit, 96 tests, Quantitative
AE-320710-1
Alpha Diagnostics
1 kit
EUR 1074
Human Anti-Zaire Ebola virus VP40 IgG ELISA Kit, 96 tests, Quantitative
AE-320720-1
Alpha Diagnostics
1 Kit
EUR 1074
Human Anti-Zaire Ebola virus VP40 IgM ELISA Kit, 96 tests, Quantitative
AE-320730-1
Alpha Diagnostics
1 Kit
EUR 1074
Recombinant (HEK) Zaire Ebola virus glycoprotein/GP (Mayinga 1976, 1-647aa, >95%)
EVGP35-R-10
Alpha Diagnostics
10 ug
EUR 416.4
Rabbit Anti-Zaire-Ebola virus nucleoprotein (Mayinga EBOV NP) protein antiserum
EVNP11-S
Alpha Diagnostics
100 ul
EUR 548.4
Rabbit Anti-Zaire Ebola virus VP40 IgG ELISA Kit, 96 tests, Quantitative
AE-320740-1
Alpha Diagnostics
1 kit
EUR 1074
Ebola hemorrhagic fever Virus (Sudan, SEBOV) One-Step PCR kit
Oneq-H703-100R
Bioingentech
100T
EUR 1932
Ebola hemorrhagic fever Virus (Sudan, SEBOV) One-Step PCR kit
Oneq-H703-150R
Bioingentech
150T
EUR 2646
Ebola hemorrhagic fever Virus (Sudan, SEBOV) One-Step PCR kit
Oneq-H703-50R
Bioingentech
50T
EUR 1410
Monkey/Chimp Anti-Zaire Ebola virus glycoprotein (GP) IgG positive control
AE-320650-PC
Alpha Diagnostics
2 ml
EUR 270
Goat Anti-Zaire Ebola virus (Mayinga) glycoprotein (GP1-637aa) IgG, purified
EVGP22-A
Alpha Diagnostics
100 ul
EUR 578.4
Mouse Monoclonal Anti-Sudan Ebola virus glycoprotein (SUDV GP) IgG, purified
SVGP22-M
Alpha Diagnostics
100 ul
EUR 578.4
Rabbit Anti-Sudan Ebola virus VP40 (Gulu/SUDV VP40/31-326aa) IgG, aff pure
SVP402-A
Alpha Diagnostics
100 ul
EUR 578.4
