Structural Foundation for a Convergent Immune Response in opposition to Ebola Virus.

Ebola virus illness is a extreme well being drawback in Africa. Vaccines that show the Zaire ebolavirus glycoprotein spike advanced are a prime part for the hassle to fight it. The V_H3-15/V_λ1-40-based class of antibodies was not too long ago found to be a standard response in people who acquired the Ebola virus vaccines.

 

• These antibodies show engaging properties, and thus seemingly contribute to the efficacy of the vaccines. Right here, we use cryo-EM to elucidate how three V_H3-15/V_λ1-40 antibodies from totally different people goal the virus and located a convergent mechanism in opposition to {a partially} conserved web site on the spike advanced.
• Our examine rationalizes the collection of the V_H3-15/V_λ1-40 germline genes for particularly concentrating on this web site and highlights Ebolavirus species-specific sequence divergences that will prohibit breadth of V_H3-15/V_λ1-40-based humoral response.
• The outcomes from this examine might assist develop improved immunization schemes and additional allow the design of immunogens that may be efficacious in opposition to a broader set of Ebolavirus species.

  

Ebola Virus antibody
10-2255	Fitzgerald	250 ug	EUR 418.8
Description: Mouse monoclonal Ebola Virus antibody

Ebola Virus Antibody
abx021693-1mg	Abbexa	1 mg	EUR 1279.2

Ebola Virus Antibody
abx021694-1mg	Abbexa	1 mg	EUR 1279.2

Ebola Virus Antibody
abx023559-1mg	Abbexa	1 mg	EUR 1195.2

Rabbit Anti-Zaire-Ebola virus nucleoprotein (Mayinga EBOV NP) protein antiserum
EVNP11-S	Alpha Diagnostics	100 ul	EUR 548.4

Recombinant Ebola virus Ebola Virus-like particles Protein, Native, Human-100ug
QP10485-100ug	EnQuireBio	100ug	EUR 925.2

Recombinant Zaire-Ebola virus nucleoprotein (Mayinga EBOV NP) protein control for Western
EVNP11-C	Alpha Diagnostics	100 ul	EUR 343.2

Ebola Virus VP40 antibody
10-2351	Fitzgerald	250 ug	EUR 457.2
Description: Mouse Monoclonal Ebola Virus VP40 antibody

Ebola Virus VP40 antibody
10-2352	Fitzgerald	250 ug	EUR 457.2
Description: Mouse Monoclonal Ebola Virus VP40 antibody

Ebola virus Nucleoprotein Antibody
abx023955-1mg	Abbexa	1 mg	EUR 1212

Ebola virus Nucleoprotein Antibody
abx023956-1mg	Abbexa	1 mg	EUR 1212

Ebola virus Nucleoprotein Antibody
abx023957-1mg	Abbexa	1 mg	EUR 1212

Ebola virus Nucleoprotein Antibody
abx023958-1mg	Abbexa	1 mg	EUR 1212

Recombinant Ebola Virus Ebola Sudan Protein Protein, Untagged, E.coli-100ug
QP11726-100ug	EnQuireBio	100ug	EUR 261.6

Recombinant Ebola Virus Ebola Sudan Protein Protein, Untagged, E.coli-1mg
QP11726-1mg	EnQuireBio	1mg	EUR 1513.2

Recombinant Ebola Virus Ebola Sudan Protein Protein, Untagged, E.coli-500ug
QP11726-500ug	EnQuireBio	500ug	EUR 795.6

Recombinant Ebola Virus Ebola Zaire Protein Protein, His, E.coli-100ug
QP11728-100ug	EnQuireBio	100ug	EUR 261.6

Recombinant Ebola Virus Ebola Zaire Protein Protein, His, E.coli-1mg
QP11728-1mg	EnQuireBio	1mg	EUR 1513.2

Recombinant Ebola Virus Ebola Zaire Protein Protein, His, E.coli-500ug
QP11728-500ug	EnQuireBio	500ug	EUR 795.6

Recombinant Ebola Virus Ebola Zaire GP Protein, His, E.coli-100ug
QP11727-100ug	EnQuireBio	100ug	EUR 261.6

Recombinant Ebola Virus Ebola Zaire GP Protein, His, E.coli-1mg
QP11727-1mg	EnQuireBio	1mg	EUR 1513.2

Recombinant Ebola Virus Ebola Zaire GP Protein, His, E.coli-500ug
QP11727-500ug	EnQuireBio	500ug	EUR 795.6

Recombinant Ebola Virus Ebola Zaire VP40 Protein, His, E.coli-100ug
QP11729-100ug	EnQuireBio	100ug	EUR 261.6

Recombinant Ebola Virus Ebola Zaire VP40 Protein, His, E.coli-1mg
QP11729-1mg	EnQuireBio	1mg	EUR 1513.2

Recombinant Ebola Virus Ebola Zaire VP40 Protein, His, E.coli-500ug
QP11729-500ug	EnQuireBio	500ug	EUR 795.6

Recombinant (E. coli) Zaire-Ebola virus nucleoprotein (Mayinga EBOV NP) protein control for Western
EVNP13-C	Alpha Diagnostics	100 ul	EUR 343.2

Mouse Anti-Zaire Ebola virus Nucleoprotein (NP) IgG ELISA Kit, 96 tests, Quantitative
AE-320500-1	Alpha Diagnostics	1 Kit	EUR 1074

Mouse Anti-Zaire Ebola virus Nucleoprotein (NP) IgM ELISA Kit, 96 tests, Quantitative
AE-320510-1	Alpha Diagnostics	1 kit	EUR 1074

Human Anti-Zaire Ebola virus Nucleoprotein (NP) IgG ELISA Kit, 96 tests, Quantitative
AE-320520-1	Alpha Diagnostics	1 Kit	EUR 1074

Human Anti-Zaire Ebola virus Nucleoprotein (NP) IgM ELISA Kit, 96 tests, Quantitative
AE-320530-1	Alpha Diagnostics	1 Kit	EUR 1074

Rabbit Anti-Zaire Ebola virus Nucleoprotein (NP) IgG ELISA Kit, 96 tests, Quantitative
AE-320540-1	Alpha Diagnostics	1 kit	EUR 1074

Rabbit Anti-Zaire Ebola virus nucleoprotein (EBOV NP, 1-739/DNA vaccine) IgG
EVNP13-A	Alpha Diagnostics	100 ul	EUR 578.4

Ebola virus Viral Protein 40 (VP40) Antibody
abx023959-1mg	Abbexa	1 mg	EUR 1212

Ebola virus Viral Protein 40 (VP40) Antibody
abx023960-1mg	Abbexa	1 mg	EUR 1212

Ebola virus Viral Protein 40 (VP40) Antibody
abx023961-1mg	Abbexa	1 mg	EUR 1212

Ebola virus Viral Protein 40 (VP40) Antibody
abx023962-1mg	Abbexa	1 mg	EUR 1212

Ebola virus Viral Protein 40 (VP40) Antibody
abx023963-1mg	Abbexa	1 mg	EUR 1212

Ebola virus Viral Protein 40 (VP40) Antibody
abx023964-1mg	Abbexa	1 mg	EUR 1212

Monkey/Chimp Anti-Zaire Ebola virus Nucleoprotein (NP) IgG ELISA Kit, 96 tests, Quantitative
AE-320550-1	Alpha Diagnostics	1 kit	EUR 1074

Monkey/Chimp Anti-Zaire Ebola virus Nucleoprotein (NP) IgM ELISA Kit, 96 tests, Quantitative
AE-320560-1	Alpha Diagnostics	1 kit	EUR 1074

Rabbit Anti-Zaire-Ebola virus nucleoprotein (Gulu, EBOV NP/630-738aa) IgG, aff pure
EVNP17-A	Alpha Diagnostics	100 ul	EUR 578.4

Recombinant (E.coli) Sudan Ebola virus nucleoprotein (SUDV NP) (Uganda, 630-738aa, his-tag, >95%)
SVNP27-R-10	Alpha Diagnostics	10 ug	EUR 416.4

Recombinant (E.coli) Sudan Ebola virus nucleoprotein (SUDV NP) (Uganda, 630-738aa, his-tag, >95%)
SVNP27-R-100	Alpha Diagnostics	100 ug	EUR 2617.2

Recombinant (E.coli) Sudan Ebola virus nucleoprotein (SUDV NP) (Gulu, 361-738aa, his-tag, >95%)
SVNP28-R-10	Alpha Diagnostics	10 ug	EUR 416.4

Recombinant (E.coli) Sudan Ebola virus nucleoprotein (SUDV NP) (Gulu, 361-738aa, his-tag, >95%)
SVNP28-R-100	Alpha Diagnostics	100 ug	EUR 2617.2

Recombinant (E.coli) Sudan Ebola virus nucleoprotein (SUDV NP) (Uganda, 1-738aa, his-tag, >95%)
SVNP29-R-10	Alpha Diagnostics	10 ug	EUR 416.4

Recombinant (E.coli) Sudan Ebola virus nucleoprotein (SUDV NP) (Uganda, 1-738aa, his-tag, >95%)
SVNP29-R-100	Alpha Diagnostics	100 ug	EUR 2617.2

Influenza B virus Nucleoprotein (NP)
1-CSB-BP361330IJK	Cusabio	EUR 1008.00 EUR 398.40 EUR 2604.00 EUR 1362.00 EUR 1894.80 EUR 564.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Influenza B virus Nucleoprotein(NP) expressed in Baculovirus

Influenza A virus Nucleoprotein (NP)
1-CSB-EP302450IGF	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Influenza A virus Nucleoprotein(NP) expressed in E.coli

Influenza A virus Nucleoprotein (NP)
1-CSB-EP304802IMB	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Influenza A virus Nucleoprotein(NP) expressed in E.coli

Influenza A virus Nucleoprotein (NP)
1-CSB-EP323566IEC	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Influenza A virus Nucleoprotein(NP) expressed in E.coli

Influenza A virus Nucleoprotein (NP)
1-CSB-EP324412IES	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Influenza A virus Nucleoprotein(NP) expressed in E.coli

Influenza A virus Nucleoprotein (NP)
1-CSB-EP325769IIM	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Influenza A virus Nucleoprotein(NP) expressed in E.coli

Influenza A virus Nucleoprotein (NP)
1-CSB-EP325878IMI	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Influenza A virus Nucleoprotein(NP) expressed in E.coli

Influenza A virus Nucleoprotein (NP)
1-CSB-EP326264IFQ	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Influenza A virus Nucleoprotein(NP) expressed in E.coli

Influenza A virus Nucleoprotein (NP)
1-CSB-EP333131IEB	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Influenza A virus Nucleoprotein(NP) expressed in E.coli

Influenza A virus Nucleoprotein (NP)
1-CSB-EP356056IFZ	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Influenza A virus Nucleoprotein(NP) expressed in E.coli

Influenza B virus Nucleoprotein (NP)
1-CSB-EP356318IJU(F1)	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Influenza B virus Nucleoprotein(NP) expressed in E.coli

Influenza B virus Nucleoprotein (NP)
1-CSB-EP356318IJU	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Influenza B virus Nucleoprotein(NP) ,partial expressed in E.coli

Influenza B virus Nucleoprotein (NP)
1-CSB-EP361330IJK	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Influenza B virus Nucleoprotein(NP) expressed in E.coli

Influenza A virus Nucleoprotein (NP)
1-CSB-EP529597IMP	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Influenza A virus Nucleoprotein(NP) expressed in E.coli

Influenza A virus Nucleoprotein (NP)
1-CSB-EP630945IFY	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Influenza A virus Nucleoprotein(NP) expressed in E.coli

Influenza A virus Nucleoprotein (NP)
1-CSB-YP325878IMI	Cusabio	EUR 814.80 EUR 402.00 EUR 2606.40 EUR 1261.20 EUR 1730.40 EUR 522.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Influenza A virus Nucleoprotein(NP) expressed in Yeast

Influenza A virus Nucleoprotein (NP)
1-CSB-YP356056IFZ	Cusabio	EUR 814.80 EUR 402.00 EUR 2606.40 EUR 1261.20 EUR 1730.40 EUR 522.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Influenza A virus Nucleoprotein(NP) expressed in Yeast

Influenza B virus Nucleoprotein (NP)
1-CSB-YP356318IJU	Cusabio	EUR 814.80 EUR 402.00 EUR 2606.40 EUR 1261.20 EUR 1730.40 EUR 522.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Influenza B virus Nucleoprotein(NP) ,partial expressed in Yeast

Influenza B virus Nucleoprotein (NP)
1-CSB-YP361330IJK	Cusabio	EUR 814.80 EUR 402.00 EUR 2606.40 EUR 1261.20 EUR 1730.40 EUR 522.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Influenza B virus Nucleoprotein(NP) expressed in Yeast

Ebola virus-like particles (EBOV VLP)
0550-001	IBT Bioservices	1mg	EUR 662.4
Description: Related to: Filoviruses Applications: WB

Mouse antibody for Ebola virus VP40
2611	Virostat	100 ug	EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.

Mouse antibody for Ebola virus VP40
2612	Virostat	100 ug	EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.

Mouse antibody for Ebola virus VP40
2613	Virostat	100 ug	EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.

Mouse antibody for Ebola virus VP40
2616	Virostat	100 ug	EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.

QPCR Kit RNA Zaire ebola virus
MOL6386	Scientific Laboratory Supplies	EACH	EUR 839.04

QPCR Kit RNA Reston ebola virus
MOL6390	Scientific Laboratory Supplies	EACH	EUR 788.88

QPCR Kit RNA Sudan Ebola virus
MOL6398	Scientific Laboratory Supplies	EACH	EUR 788.88

Recombinant (E.coli) Zaire Ebola virus nucleoprotein (EBOV NP) (full length, his-tag, 82 kda), purified
EVNP15-R-10	Alpha Diagnostics	10 ug	EUR 416.4

Recombinant (E.coli) Zaire Ebola virus nucleoprotein (EBOV NP) (full length, his-tag, 82 kda), purified
EVNP15-R-100	Alpha Diagnostics	100 ug	EUR 2617.2

Recombinant (E.coli) Zaire Ebola virus nucleoprotein (EBOV NP) (full length, his-tag, 82 kda)-Biotin Conjugate
EVNP15-BTN	Alpha Diagnostics	100 ul	EUR 489.6

Mouse anti-Ebola Virus Nucleoprotein monoclonal antibody
DMAB-A005	Creative Diagnostics	1 mg	EUR 1552.8

Mouse anti-Ebola Virus Nucleoprotein monoclonal antibody
DMAB-A006	Creative Diagnostics	1 mg	EUR 1552.8

Mouse antibody for Mumps virus NP
6007	Virostat	100 ug	EUR 386.26
Description: This is purified Mouse monoclonal antibody against Mumps virus NP for WB, ELISA.

Mouse antibody for Mumps virus NP
6008	Virostat	100 ug	EUR 386.26
Description: This is purified Mouse monoclonal antibody against Mumps virus NP for WB, ELISA.

Custom Testing of Samples for Antibodies (IgA/IgG/IgM) to Ebola/Marburg Virus Protein (NP/GP/VP40) ELISA
AE-320520-CUX	Alpha Diagnostics	Custom	Ask for price

Ebola hemorrhagic fever Virus (Sudan, SEBOV) PCR kit
PCR-H703-48R	Bioingentech	50T	EUR 987.6

Ebola hemorrhagic fever Virus (Sudan, SEBOV) PCR kit
PCR-H703-96R	Bioingentech	100T	EUR 1335.6

Mouse antibody for Influenza B virus NP
1131	Virostat	100 ug	EUR 386.26
Description: This is purified Mouse monoclonal antibody against Influenza B virus NP for WB, ELISA.

Mouse antibody for Influenza A virus NP
1331	Virostat	100 ug	EUR 386.26
Description: This is purified Mouse monoclonal antibody against Influenza A virus NP for WB, ELISA.

Mouse antibody for Influenza A virus NP
1341	Virostat	100 ug	EUR 386.26
Description: This is purified Mouse monoclonal antibody against Influenza A virus NP for WB, ELISA.

Mouse antibody for Influenza A virus NP
1361	Virostat	100 ug	EUR 386.26
Description: This is purified Mouse monoclonal antibody against Influenza A virus NP for WB, ELISA.

PREVAIL I Cluster Vaccination Research With rVSVΔG-ZEBOV-GP as A part of a Public Well being Response in Liberia.

In November 2015, a 15-year-old boy acquired a prognosis of Ebola virus illness (EVD) on the John F. Kennedy Medical Middle in Monrovia, Liberia. Two extra members of the family acquired a prognosis of EVD. The protocol for a part 2 placebo-controlled trial of two Ebola vaccines was amended and accredited; in four days, a single-arm cluster vaccination trial utilizing the Merck rVSVΔG-ZEBOV-GP vaccine was initiated.

Right here, we consider the protection and immunogenicity of the vaccine and focus on challenges for its implementation in a small Ebola outbreak. We performed a hoop vaccination examine amongst contacts and contacts of shut contacts of EVD circumstances a in Monrovia. Members had been evaluated 1 and 6 months after vaccination.

Amongst 650 shut contacts and contacts of shut contacts of EVD circumstances, 210 (32%) consented and had been vaccinated with rVSVΔG-ZEBOV-GP. Of these vaccinated, 189 (90%) attended the month 1 follow-up go to; 166 (79%) attended the month 6 go to. No severe opposed occasions had been reported.

Amongst 88 individuals with out an elevated antibody stage at baseline, 77.3% (95% confidence interval, 68.5-86.1) had an antibody response at 1 month. The Merck rVSVΔG-ZEBOV-GP vaccine appeared to be secure and immunogenic among the many vaccinated people.

Nevertheless, fewer than one third of eligible people consented to vaccination. These information could assist information implementation selections for of cluster vaccination packages in an Ebola cluster outbreak response state of affairs

Design of Fusion Proteins for Environment friendly and Soluble Manufacturing of Immunogenic Ebola Virus Glycoprotein in Escherichia coli.

The Ebola hemorrhagic fever brought on by Ebola virus is a particularly harmful illness, and efficient therapeutic brokers are nonetheless missing. Platforms for the environment friendly manufacturing of vaccines are essential to make sure fast response in opposition to an Ebola virus outbreak.

Ebola virus glycoprotein (EbolaGP) on the virion floor is answerable for membrane binding and virus entry, thus turning into the important thing goal for vaccine improvement. Nevertheless, heterologous expression of this protein nonetheless faces engineering challenges equivalent to low manufacturing ranges and insoluble aggregation. Right here, the authors design and evaluate numerous fusion methods, attaching nice significance to the solubility-enhancing impact, and tag elimination course of.

It’s discovered {that a} C-terminal intein-based tag vastly enhances the solubility of EbolaGP and permits one-step chromatographic purification of the untagged EbolaGP by means of thiol-catalyzed self-cleavage. The purified untagged EbolaGP alone or with Freund’s adjuvant are extremely immunogenic, as confirmed in a mouse mannequin.

Consequently, the current examine places ahead a brand new technique for the environment friendly and soluble expression of untagged immunogenic EbolaGP. The intein-based protein fusion method could also be of significance for the large-scale manufacturing of the Ebola virus subunit vaccine.