Structural Foundation for a Convergent Immune Response in opposition to Ebola Virus.

Ebola virus illness is a extreme well being drawback in Africa. Vaccines that show the Zaire ebolavirus glycoprotein spike advanced are a prime part for the hassle to fight it. The V_H3-15/V_λ1-40-based class of antibodies was not too long ago found to be a standard response in people who acquired the Ebola virus vaccines.

 

• These antibodies show engaging properties, and thus seemingly contribute to the efficacy of the vaccines. Right here, we use cryo-EM to elucidate how three V_H3-15/V_λ1-40 antibodies from totally different people goal the virus and located a convergent mechanism in opposition to {a partially} conserved web site on the spike advanced.
• Our examine rationalizes the collection of the V_H3-15/V_λ1-40 germline genes for particularly concentrating on this web site and highlights Ebolavirus species-specific sequence divergences that will prohibit breadth of V_H3-15/V_λ1-40-based humoral response.
• The outcomes from this examine might assist develop improved immunization schemes and additional allow the design of immunogens that may be efficacious in opposition to a broader set of Ebolavirus species.

  

Recombinant Ebola Virus Ebola Zaire Protein Protein, His, E.coli-500ug
QP11728-500ug	EnQuireBio	500ug	EUR 795.6

Recombinant Ebola Virus Ebola Sudan Protein Protein, Untagged, E.coli-1mg
QP11726-1mg	EnQuireBio	1mg	EUR 1513.2

Recombinant Ebola Virus Ebola Zaire GP Protein, His, E.coli-1mg
QP11727-1mg	EnQuireBio	1mg	EUR 1513.2

Recombinant Ebola Virus Ebola Sudan Protein Protein, Untagged, E.coli-100ug
QP11726-100ug	EnQuireBio	100ug	EUR 261.6

Recombinant Ebola Virus Ebola Sudan Protein Protein, Untagged, E.coli-500ug
QP11726-500ug	EnQuireBio	500ug	EUR 795.6

Recombinant Ebola Virus Ebola Zaire GP Protein, His, E.coli-100ug
QP11727-100ug	EnQuireBio	100ug	EUR 261.6

Recombinant Ebola Virus Ebola Zaire GP Protein, His, E.coli-500ug
QP11727-500ug	EnQuireBio	500ug	EUR 795.6

Recombinant Ebola Virus Ebola Zaire VP40 Protein, His, E.coli-1mg
QP11729-1mg	EnQuireBio	1mg	EUR 1513.2

Recombinant Ebola Virus Ebola Zaire VP40 Protein, His, E.coli-100ug
QP11729-100ug	EnQuireBio	100ug	EUR 261.6

Recombinant Ebola Virus Ebola Zaire VP40 Protein, His, E.coli-500ug
QP11729-500ug	EnQuireBio	500ug	EUR 795.6

Ebola Virus antibody
10-2253	Fitzgerald	250 ug	EUR 363.6
Description: Mouse monoclonal Ebola Virus antibody

Ebola Virus antibody
10-2254	Fitzgerald	250 ug	EUR 354
Description: Mouse monoclonal Ebola Virus antibody

Ebola Virus antibody
10-2255	Fitzgerald	250 ug	EUR 418.8
Description: Mouse monoclonal Ebola Virus antibody

Ebola Virus Antibody
abx021693-1mg	Abbexa	1 mg	EUR 1279.2

Ebola Virus Antibody
abx021694-1mg	Abbexa	1 mg	EUR 1279.2

Ebola Virus Antibody
abx023559-1mg	Abbexa	1 mg	EUR 1195.2

Ebola virus Viral Protein 40 (VP40) Antibody
abx023959-1mg	Abbexa	1 mg	EUR 1212

Ebola virus Viral Protein 40 (VP40) Antibody
abx023960-1mg	Abbexa	1 mg	EUR 1212

Ebola virus Viral Protein 40 (VP40) Antibody
abx023961-1mg	Abbexa	1 mg	EUR 1212

Ebola virus Viral Protein 40 (VP40) Antibody
abx023962-1mg	Abbexa	1 mg	EUR 1212

Ebola virus Viral Protein 40 (VP40) Antibody
abx023963-1mg	Abbexa	1 mg	EUR 1212

Ebola virus Viral Protein 40 (VP40) Antibody
abx023964-1mg	Abbexa	1 mg	EUR 1212

Rabbit Anti-Sudan Ebola virus Nucleoprotein (SUDV NP) IgG
SVNP25-A	Alpha Diagnostics	100 ul	EUR 578.4

Ebola Virus VP40 antibody
10-2351	Fitzgerald	250 ug	EUR 457.2
Description: Mouse Monoclonal Ebola Virus VP40 antibody

Ebola Virus VP40 antibody
10-2352	Fitzgerald	250 ug	EUR 457.2
Description: Mouse Monoclonal Ebola Virus VP40 antibody

Ebola virus Nucleoprotein Antibody
abx023955-1mg	Abbexa	1 mg	EUR 1212

Ebola virus Nucleoprotein Antibody
abx023956-1mg	Abbexa	1 mg	EUR 1212

Ebola virus Nucleoprotein Antibody
abx023957-1mg	Abbexa	1 mg	EUR 1212

Ebola virus Nucleoprotein Antibody
abx023958-1mg	Abbexa	1 mg	EUR 1212

QPCR Kit RNA Zaire ebola virus
MOL6386	Scientific Laboratory Supplies	EACH	EUR 1045.79

QPCR Kit RNA Sudan Ebola virus
MOL6398	Scientific Laboratory Supplies	EACH	EUR 788.88

QPCR Kit RNA Reston ebola virus
MOL6390	Scientific Laboratory Supplies	EACH	EUR 788.88

Mouse antibody for Ebola virus VP40
2611	Virostat	100 ug	EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.

Mouse antibody for Ebola virus VP40
2612	Virostat	100 ug	EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.

Mouse antibody for Ebola virus VP40
2613	Virostat	100 ug	EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.

Mouse antibody for Ebola virus VP40
2614	Virostat	100 ug	EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.

Mouse antibody for Ebola virus VP40
2615	Virostat	100 ug	EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.

Mouse antibody for Ebola virus VP40
2616	Virostat	100 ug	EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus VP40 for WB, ELISA.

Ebola virus-like particles (EBOV VLP)
0550-001	IBT Bioservices	1mg	EUR 662.4
Description: Related to: Filoviruses Applications: WB

Rabbit Anti-Zaire Ebola virus nucleoprotein (EBOV NP, 1-739/DNA vaccine) IgG
EVNP13-A	Alpha Diagnostics	100 ul	EUR 578.4

Custom Testing of Samples for Antibodies (IgA/IgG/IgM) to Ebola/Marburg Virus Protein (NP/GP/VP40) ELISA
AE-320520-CUX	Alpha Diagnostics	Custom	Ask for price

Mouse Anti-Zaire Ebola virus Nucleoprotein (NP) IgG ELISA Kit, 96 tests, Quantitative
AE-320500-1	Alpha Diagnostics	1 Kit	EUR 1074

Mouse Anti-Zaire Ebola virus Nucleoprotein (NP) IgM ELISA Kit, 96 tests, Quantitative
AE-320510-1	Alpha Diagnostics	1 kit	EUR 1074

Human Anti-Zaire Ebola virus Nucleoprotein (NP) IgG ELISA Kit, 96 tests, Quantitative
AE-320520-1	Alpha Diagnostics	1 Kit	EUR 1074

Human Anti-Zaire Ebola virus Nucleoprotein (NP) IgM ELISA Kit, 96 tests, Quantitative
AE-320530-1	Alpha Diagnostics	1 Kit	EUR 1074

Rabbit Anti-Zaire Ebola virus Nucleoprotein (NP) IgG ELISA Kit, 96 tests, Quantitative
AE-320540-1	Alpha Diagnostics	1 kit	EUR 1074

Recombinant (E.coli) Sudan Ebola virus nucleoprotein (SUDV NP) (Gulu, 361-738aa, his-tag, >95%)
SVNP28-R-10	Alpha Diagnostics	10 ug	EUR 416.4

Recombinant (E.coli) Sudan Ebola virus nucleoprotein (SUDV NP) (Gulu, 361-738aa, his-tag, >95%)
SVNP28-R-100	Alpha Diagnostics	100 ug	EUR 2617.2

Recombinant (E.coli) Sudan Ebola virus nucleoprotein (SUDV NP) (Uganda, 630-738aa, his-tag, >95%)
SVNP27-R-10	Alpha Diagnostics	10 ug	EUR 416.4

Recombinant (E.coli) Sudan Ebola virus nucleoprotein (SUDV NP) (Uganda, 630-738aa, his-tag, >95%)
SVNP27-R-100	Alpha Diagnostics	100 ug	EUR 2617.2

Recombinant (E.coli) Sudan Ebola virus nucleoprotein (SUDV NP) (Uganda, 1-738aa, his-tag, >95%)
SVNP29-R-10	Alpha Diagnostics	10 ug	EUR 416.4

Recombinant (E.coli) Sudan Ebola virus nucleoprotein (SUDV NP) (Uganda, 1-738aa, his-tag, >95%)
SVNP29-R-100	Alpha Diagnostics	100 ug	EUR 2617.2

Monkey/Chimp Anti-Zaire Ebola virus Nucleoprotein (NP) IgG ELISA Kit, 96 tests, Quantitative
AE-320550-1	Alpha Diagnostics	1 kit	EUR 1074

Monkey/Chimp Anti-Zaire Ebola virus Nucleoprotein (NP) IgM ELISA Kit, 96 tests, Quantitative
AE-320560-1	Alpha Diagnostics	1 kit	EUR 1074

Recombinant (E.coli) Zaire Ebola virus nucleoprotein (EBOV NP) (full length, his-tag, 82 kda), purified
EVNP15-R-10	Alpha Diagnostics	10 ug	EUR 416.4

Recombinant (E.coli) Zaire Ebola virus nucleoprotein (EBOV NP) (full length, his-tag, 82 kda), purified
EVNP15-R-100	Alpha Diagnostics	100 ug	EUR 2617.2

Recombinant (E. coli) Bundibugyo Ebola virus VP40 protein (Uganda/2007/1-326aa, His-tag at NT)
BVP401-R-10	Alpha Diagnostics	10 ug	EUR 416.4

Recombinant (E.coli) Zaire Ebola virus nucleoprotein (EBOV NP) (full length, his-tag, 82 kda)-Biotin Conjugate
EVNP15-BTN	Alpha Diagnostics	100 ul	EUR 489.6

Ebola hemorrhagic fever Virus (Sudan, SEBOV) PCR kit
PCR-H703-48R	Bioingentech	50T	EUR 987.6

Ebola hemorrhagic fever Virus (Sudan, SEBOV) PCR kit
PCR-H703-96R	Bioingentech	100T	EUR 1335.6

Ebola hemorrhagic fever Virus (Sudan, SEBOV) RT PCR kit
RTq-H703-100R	Bioingentech	100T	EUR 1573.2

Ebola hemorrhagic fever Virus (Sudan, SEBOV) RT PCR kit
RTq-H703-150R	Bioingentech	150T	EUR 2144.4

Ebola hemorrhagic fever Virus (Sudan, SEBOV) RT PCR kit
RTq-H703-50R	Bioingentech	50T	EUR 1155.6

Ebola NP antigen [His]
DAGA-3013	Creative Diagnostics	1mg	EUR 1092

Recombinant (E.coli) Sudan Ebola virus VP40 (Uganda,31-326aa, His-tag, >95%)
SVP407-R-10	Alpha Diagnostics	10 ug	EUR 416.4

Recombinant (E.coli) Sudan Ebola virus VP40 (Uganda,31-326aa, His-tag, >95%)
SVP407-R-100	Alpha Diagnostics	100 ug	EUR 2617.2

Recombinant (E.coli) Zaire Ebola virus nucleoprotein (EBOV NP) (H.sapiens-wt/GIN/2014/Kissidougou-C15, 630-739aa, his-tag, >95%)
EVNP16-R-10	Alpha Diagnostics	10 ug	EUR 416.4

Recombinant (E.coli) Zaire Ebola virus nucleoprotein (EBOV NP) (H.sapiens-wt/GIN/2014/Kissidougou-C15, 630-739aa , his-tag, >95%)
EVNP16-R-100	Alpha Diagnostics	100 ug	EUR 2617.2

Mouse Anti-Zaire Ebola virus VP40 IgG ELISA Kit, 96 tests, Quantitative
AE-320700-1	Alpha Diagnostics	1 kit	EUR 1074

Mouse Anti-Zaire Ebola virus VP40 IgM ELISA Kit, 96 tests, Quantitative
AE-320710-1	Alpha Diagnostics	1 kit	EUR 1074

Human Anti-Zaire Ebola virus VP40 IgG ELISA Kit, 96 tests, Quantitative
AE-320720-1	Alpha Diagnostics	1 Kit	EUR 1074

Human Anti-Zaire Ebola virus VP40 IgM ELISA Kit, 96 tests, Quantitative
AE-320730-1	Alpha Diagnostics	1 Kit	EUR 1074

Recombinant (HEK) Zaire Ebola virus glycoprotein/GP (Mayinga 1976, 1-647aa, >95%)
EVGP35-R-10	Alpha Diagnostics	10 ug	EUR 416.4

Monkey/Chimp Anti-Zaire Ebola virus glycoprotein (GP) IgG positive control
AE-320650-PC	Alpha Diagnostics	2 ml	EUR 270

Goat Anti-Zaire Ebola virus (Mayinga) glycoprotein (GP1-637aa) IgG, purified
EVGP22-A	Alpha Diagnostics	100 ul	EUR 578.4

Mouse Monoclonal Anti-Sudan Ebola virus glycoprotein (SUDV GP) IgG, purified
SVGP22-M	Alpha Diagnostics	100 ul	EUR 578.4

Rabbit Anti-Zaire Ebola virus VP40 IgG ELISA Kit, 96 tests, Quantitative
AE-320740-1	Alpha Diagnostics	1 kit	EUR 1074

Zaire Ebola Virus Glycoprotein (EBOV GP antigen) ELISA Kit, 48 tests, Quantitative
AE-320800-48	Alpha Diagnostics	1 kit	EUR 1074

Zaire Ebola Virus Glycoprotein (EBOV GP antigen) ELISA Kit, 96 tests, Quantitative
AE-320800-96	Alpha Diagnostics	1 kit	EUR 1804.8

Ebola hemorrhagic fever Virus (Sudan, SEBOV) One-Step PCR kit
Oneq-H703-100R	Bioingentech	100T	EUR 1932

Ebola hemorrhagic fever Virus (Sudan, SEBOV) One-Step PCR kit
Oneq-H703-150R	Bioingentech	150T	EUR 2646

Ebola hemorrhagic fever Virus (Sudan, SEBOV) One-Step PCR kit
Oneq-H703-50R	Bioingentech	50T	EUR 1410

Rabbit Anti-Sudan Ebola virus VP40 (Gulu/SUDV VP40/31-326aa) IgG, aff pure
SVP402-A	Alpha Diagnostics	100 ul	EUR 578.4

Mouse Monoclonal Anti-Anti-Zaire Ebola virus glycoprotein (EBOV GP) IgG, purified
EVGP20-M	Alpha Diagnostics	100 ul	EUR 578.4

Horse Anti-Zaire Ebola virus glycoprotein IgG ELISA Kit, 96 tests, Quantitative
AE-320690-1	Alpha Diagnostics	1 Kit	EUR 1220.4

Rabbit Anti-Sudan Ebola virus glycoprotein (sf9, SUDV GP 1-637aa) IgG purified
SVGP21-A	Alpha Diagnostics	100 ul	EUR 578.4

Monkey/Chimp Anti-Zaire Ebola virus VP40 IgG ELISA Kit, 96 tests, Quantitative
AE-320750-1	Alpha Diagnostics	1 kit	EUR 1074

Monkey/Chimp Anti-Zaire Ebola virus VP40 IgM ELISA Kit, 96 tests, Quantitative
AE-320760-1	Alpha Diagnostics	1 kit	EUR 1074

PREVAIL I Cluster Vaccination Research With rVSVΔG-ZEBOV-GP as A part of a Public Well being Response in Liberia.

In November 2015, a 15-year-old boy acquired a prognosis of Ebola virus illness (EVD) on the John F. Kennedy Medical Middle in Monrovia, Liberia. Two extra members of the family acquired a prognosis of EVD. The protocol for a part 2 placebo-controlled trial of two Ebola vaccines was amended and accredited; in four days, a single-arm cluster vaccination trial utilizing the Merck rVSVΔG-ZEBOV-GP vaccine was initiated.

Right here, we consider the protection and immunogenicity of the vaccine and focus on challenges for its implementation in a small Ebola outbreak. We performed a hoop vaccination examine amongst contacts and contacts of shut contacts of EVD circumstances a in Monrovia. Members had been evaluated 1 and 6 months after vaccination.

Amongst 650 shut contacts and contacts of shut contacts of EVD circumstances, 210 (32%) consented and had been vaccinated with rVSVΔG-ZEBOV-GP. Of these vaccinated, 189 (90%) attended the month 1 follow-up go to; 166 (79%) attended the month 6 go to. No severe opposed occasions had been reported.

Amongst 88 individuals with out an elevated antibody stage at baseline, 77.3% (95% confidence interval, 68.5-86.1) had an antibody response at 1 month. The Merck rVSVΔG-ZEBOV-GP vaccine appeared to be secure and immunogenic among the many vaccinated people.

Nevertheless, fewer than one third of eligible people consented to vaccination. These information could assist information implementation selections for of cluster vaccination packages in an Ebola cluster outbreak response state of affairs

Design of Fusion Proteins for Environment friendly and Soluble Manufacturing of Immunogenic Ebola Virus Glycoprotein in Escherichia coli.

The Ebola hemorrhagic fever brought on by Ebola virus is a particularly harmful illness, and efficient therapeutic brokers are nonetheless missing. Platforms for the environment friendly manufacturing of vaccines are essential to make sure fast response in opposition to an Ebola virus outbreak.

Ebola virus glycoprotein (EbolaGP) on the virion floor is answerable for membrane binding and virus entry, thus turning into the important thing goal for vaccine improvement. Nevertheless, heterologous expression of this protein nonetheless faces engineering challenges equivalent to low manufacturing ranges and insoluble aggregation. Right here, the authors design and evaluate numerous fusion methods, attaching nice significance to the solubility-enhancing impact, and tag elimination course of.

It’s discovered {that a} C-terminal intein-based tag vastly enhances the solubility of EbolaGP and permits one-step chromatographic purification of the untagged EbolaGP by means of thiol-catalyzed self-cleavage. The purified untagged EbolaGP alone or with Freund’s adjuvant are extremely immunogenic, as confirmed in a mouse mannequin.

Consequently, the current examine places ahead a brand new technique for the environment friendly and soluble expression of untagged immunogenic EbolaGP. The intein-based protein fusion method could also be of significance for the large-scale manufacturing of the Ebola virus subunit vaccine.