Structural Foundation for a Convergent Immune Response in opposition to Ebola Virus.

Ebola virus illness is a extreme well being drawback in Africa. Vaccines that show the Zaire ebolavirus glycoprotein spike advanced are a prime part for the hassle to fight it. The V_H3-15/V_λ1-40-based class of antibodies was not too long ago found to be a standard response in people who acquired the Ebola virus vaccines.

 

• These antibodies show engaging properties, and thus seemingly contribute to the efficacy of the vaccines. Right here, we use cryo-EM to elucidate how three V_H3-15/V_λ1-40 antibodies from totally different people goal the virus and located a convergent mechanism in opposition to {a partially} conserved web site on the spike advanced.
• Our examine rationalizes the collection of the V_H3-15/V_λ1-40 germline genes for particularly concentrating on this web site and highlights Ebolavirus species-specific sequence divergences that will prohibit breadth of V_H3-15/V_λ1-40-based humoral response.
• The outcomes from this examine might assist develop improved immunization schemes and additional allow the design of immunogens that may be efficacious in opposition to a broader set of Ebolavirus species.

  

MAb to Ebola Virus NP
MBS313479-5x1mg	MyBiosource	5x1mg	EUR 4175

MAb to Ebola Virus NP
MBS313480-1mg	MyBiosource	1mg	EUR 965

MAb to Ebola Virus NP
MBS313480-5x1mg	MyBiosource	5x1mg	EUR 4175

Ebola Virus VP40 protein
30-1837	Fitzgerald	100 ug	EUR 136
Description: Recombinant Ebola Virus VP40 Matrix protein

Ebola Virus VP40 protein
MBS5304547-01mg	MyBiosource	0.1mg	EUR 275

Ebola Virus VP40 protein
MBS5304547-5x01mg	MyBiosource	5x0.1mg	EUR 1015

Ebola virus NP recombinant antigen
8828	Virostat	100 ug	EUR 673.36
Description: This is Ebola virus NP recombinant antigen for ELISA,WB.

Ebola Virus Nucleoprotein (NP) (Zaire)
REC31728-100	The Native Antigen Company	0.1	EUR 395.48
Description: Recombinant Ebolavirus NP (Zaire) C-term produced in E. coli. Protein is ~15 kDa and contains a C-terminal His-tag.

Ebola Virus Nucleoprotein (NP) (Zaire)
REC31728-500	The Native Antigen Company	0.5	EUR 1386.75
Description: Recombinant Ebolavirus NP (Zaire) C-term produced in E. coli. Protein is ~15 kDa and contains a C-terminal His-tag.

Ebola Virus Nucleoprotein (NP) (Sudan)
REC31729-100	The Native Antigen Company	0.1	EUR 395.48
Description: Recombinant Ebolavirus NP (Sudan) C-term produced in E. coli.

Ebola Virus Nucleoprotein (NP) (Sudan)
REC31729-500	The Native Antigen Company	0.5	EUR 1386.75
Description: Recombinant Ebolavirus NP (Sudan) C-term produced in E. coli.

Recombinant Ebola virus Ebola Virus-like particles Protein, Native, Human-100ug
QP10485-100ug	EnQuireBio	100ug	EUR 925.2

Mouse antibody for Ebola virus NP
2601	Virostat	100 ug	EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus NP for WB, ELISA.

Mouse antibody for Ebola virus NP
2602	Virostat	100 ug	EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus NP for WB, ELISA.

Mouse antibody for Ebola virus NP
2603	Virostat	100 ug	EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus NP for WB, ELISA.

Mouse antibody for Ebola virus NP
2604	Virostat	100 ug	EUR 405.83
Description: This is purified Mouse monoclonal antibody against Ebola virus NP for WB, ELISA.

Ebola Virus (Sudan-NP) Antigen >95%
MBS569837-1mg	MyBiosource	1mg	EUR 795

Ebola Virus (Sudan-NP) Antigen >95%
MBS569837-5x1mg	MyBiosource	5x1mg	EUR 3370

Ebola Virus (Zaire-NP) Antigen >95%
MBS569839-1mg	MyBiosource	1mg	EUR 795

Ebola Virus (Zaire-NP) Antigen >95%
MBS569839-5x1mg	MyBiosource	5x1mg	EUR 3370

Recombinant Ebola Virus Ebola Zaire Protein Protein, His, E.coli-1mg
QP11728-1mg	EnQuireBio	1mg	EUR 1513.2

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (AP)
MBS6126138-01mL	MyBiosource	0.1(mL	EUR 1270

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (AP)
MBS6126138-5x01mL	MyBiosource	5x0.1mL	EUR 5560

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (HRP)
MBS6128611-01mL	MyBiosource	0.1(mL	EUR 1270

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (HRP)
MBS6128611-5x01mL	MyBiosource	5x0.1mL	EUR 5560

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (PE)
MBS6129232-01mL	MyBiosource	0.1(mL	EUR 1270

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (PE)
MBS6129232-5x01mL	MyBiosource	5x0.1mL	EUR 5560

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (APC)
MBS6126756-01mL	MyBiosource	0.1(mL	EUR 1270

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (APC)
MBS6126756-5x01mL	MyBiosource	5x0.1mL	EUR 5560

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (FITC)
MBS6127995-01mL	MyBiosource	0.1(mL	EUR 1270

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (FITC)
MBS6127995-5x01mL	MyBiosource	5x0.1mL	EUR 5560

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (AP)
MBS6260423-01mL	MyBiosource	0.1mL	EUR 745

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (AP)
MBS6260423-5x01mL	MyBiosource	5x0.1mL	EUR 3195

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (FITC)
MBS6261953-01mL	MyBiosource	0.1mL	EUR 745

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (FITC)
MBS6261953-5x01mL	MyBiosource	5x0.1mL	EUR 3195

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (HRP)
MBS6262463-01mL	MyBiosource	0.1mL	EUR 745

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (HRP)
MBS6262463-5x01mL	MyBiosource	5x0.1mL	EUR 3195

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (PE)
MBS6262974-01mL	MyBiosource	0.1mL	EUR 745

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (PE)
MBS6262974-5x01mL	MyBiosource	5x0.1mL	EUR 3195

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (APC)
MBS6260934-01mL	MyBiosource	0.1mL	EUR 745

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (APC)
MBS6260934-5x01mL	MyBiosource	5x0.1mL	EUR 3195

Ebola Virus (Zaire & Sudan-NP) Antibody
MBS5680215-1mg	MyBiosource	1mg	EUR 585

Ebola Virus (Zaire & Sudan-NP) Antibody
MBS5680215-5x1mg	MyBiosource	5x1mg	EUR 2440

Ebola Virus (Zaire & Sudan-NP) Antibody
MBS5680216-1mg	MyBiosource	1mg	EUR 585

Ebola Virus (Zaire & Sudan-NP) Antibody
MBS5680216-5x1mg	MyBiosource	5x1mg	EUR 2440

Recombinant Ebola Virus Ebola Zaire Protein Protein, His, E.coli-100ug
QP11728-100ug	EnQuireBio	100ug	EUR 261.6

Recombinant Ebola Virus Ebola Zaire Protein Protein, His, E.coli-500ug
QP11728-500ug	EnQuireBio	500ug	EUR 795.6

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (Biotin)
MBS6127376-01mL	MyBiosource	0.1(mL	EUR 1270

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (Biotin)
MBS6127376-5x01mL	MyBiosource	5x0.1mL	EUR 5560

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (Biotin)
MBS6261445-01mL	MyBiosource	0.1mL	EUR 745

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (Biotin)
MBS6261445-5x01mL	MyBiosource	5x0.1mL	EUR 3195

Ebola Virus Nucleoprotein (EBOV NP) Antibody
abx201313-100l	Abbexa	100 µl	EUR 450

Ebola Virus Nucleoprotein (EBOV NP) Antibody
abx201313-50l	Abbexa	50 µl	EUR 312.5

Ebola Virus Nucleoprotein (EBOV NP) Antibody
abx201314-100l	Abbexa	100 µl	EUR 450

Ebola Virus Nucleoprotein (EBOV NP) Antibody
abx201314-50l	Abbexa	50 µl	EUR 312.5

Ebola Virus Nucleoprotein (EBOV NP) Antibody
abx201315-100l	Abbexa	100 µl	EUR 450

Ebola Virus Nucleoprotein (EBOV NP) Antibody
abx201315-50l	Abbexa	50 µl	EUR 312.5

Ebola Virus Nucleoprotein (EBOV NP) Antibody
abx201316-100l	Abbexa	100 µl	EUR 450

Ebola Virus Nucleoprotein (EBOV NP) Antibody
abx201316-50l	Abbexa	50 µl	EUR 312.5

Ebola Virus Nucleoprotein (EBOV NP) Antibody
abx201317-100l	Abbexa	100 µl	EUR 450

Ebola Virus Nucleoprotein (EBOV NP) Antibody
abx201317-50l	Abbexa	50 µl	EUR 312.5

Ebola Virus Nucleoprotein (EBOV NP) Antibody
abx201320-100l	Abbexa	100 µl	EUR 325

Ebola Virus Nucleoprotein (EBOV NP) Antibody
abx201320-50l	Abbexa	50 µl	EUR 225

Ebola Virus Nucleoprotein (EBOV NP) Antibody
abx201325-100l	Abbexa	100 µl	EUR 450

Ebola Virus Nucleoprotein (EBOV NP) Antibody
abx201325-50l	Abbexa	50 µl	EUR 312.5

Ebola Virus Nucleoprotein (EBOV NP) Antibody
abx201326-100l	Abbexa	100 µl	EUR 450

Ebola Virus Nucleoprotein (EBOV NP) Antibody
abx201326-50l	Abbexa	50 µl	EUR 312.5

Ebola Virus Nucleoprotein (EBOV NP) Antibody
abx201327-100l	Abbexa	100 µl	EUR 450

Ebola Virus Nucleoprotein (EBOV NP) Antibody
abx201327-50l	Abbexa	50 µl	EUR 312.5

Recombinant Ebola Virus Ebola Sudan Protein Protein, Untagged, E.coli-1mg
QP11726-1mg	EnQuireBio	1mg	EUR 1513.2

Recombinant Ebola Virus Ebola Sudan Protein Protein, Untagged, E.coli-100ug
QP11726-100ug	EnQuireBio	100ug	EUR 261.6

Recombinant Ebola Virus Ebola Sudan Protein Protein, Untagged, E.coli-500ug
QP11726-500ug	EnQuireBio	500ug	EUR 795.6

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (MaxLight 490)
MBS6263996-01mL	MyBiosource	0.1mL	EUR 745

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (MaxLight 490)
MBS6263996-5x01mL	MyBiosource	5x0.1mL	EUR 3195

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (MaxLight 550)
MBS6264507-01mL	MyBiosource	0.1mL	EUR 745

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (MaxLight 550)
MBS6264507-5x01mL	MyBiosource	5x0.1mL	EUR 3195

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (MaxLight 650)
MBS6265018-01mL	MyBiosource	0.1mL	EUR 745

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (MaxLight 650)
MBS6265018-5x01mL	MyBiosource	5x0.1mL	EUR 3195

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (MaxLight 750)
MBS6265529-01mL	MyBiosource	0.1mL	EUR 745

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (MaxLight 750)
MBS6265529-5x01mL	MyBiosource	5x0.1mL	EUR 3195

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (MaxLight 405)
MBS6263485-01mL	MyBiosource	0.1mL	EUR 745

Ebola Virus (Ebolavirus, EHF, Ebola Virus Disease, EV, EVD, EBOV, Ebola, Ebola Hemorrhagic Fever, Ebola Virus (Zaire)) (MaxLight 405)
MBS6263485-5x01mL	MyBiosource	5x0.1mL	EUR 3195

Ebola Virus
MBS601517-1mg	MyBiosource	1mg	EUR 1070

Ebola Virus
MBS601517-5x1mg	MyBiosource	5x1mg	EUR 4670

Ebola Virus
MBS601619-05mg	MyBiosource	0.5mg	EUR 640

Ebola Virus
MBS601619-1mg	MyBiosource	1mg	EUR 1085

Ebola Virus
MBS601619-5x1mg	MyBiosource	5x1mg	EUR 4730

Ebola Virus
MBS319682-1mg	MyBiosource	1mg	EUR 785

PREVAIL I Cluster Vaccination Research With rVSVΔG-ZEBOV-GP as A part of a Public Well being Response in Liberia.

In November 2015, a 15-year-old boy acquired a prognosis of Ebola virus illness (EVD) on the John F. Kennedy Medical Middle in Monrovia, Liberia. Two extra members of the family acquired a prognosis of EVD. The protocol for a part 2 placebo-controlled trial of two Ebola vaccines was amended and accredited; in four days, a single-arm cluster vaccination trial utilizing the Merck rVSVΔG-ZEBOV-GP vaccine was initiated.

Right here, we consider the protection and immunogenicity of the vaccine and focus on challenges for its implementation in a small Ebola outbreak. We performed a hoop vaccination examine amongst contacts and contacts of shut contacts of EVD circumstances a in Monrovia. Members had been evaluated 1 and 6 months after vaccination.

Amongst 650 shut contacts and contacts of shut contacts of EVD circumstances, 210 (32%) consented and had been vaccinated with rVSVΔG-ZEBOV-GP. Of these vaccinated, 189 (90%) attended the month 1 follow-up go to; 166 (79%) attended the month 6 go to. No severe opposed occasions had been reported.

Amongst 88 individuals with out an elevated antibody stage at baseline, 77.3% (95% confidence interval, 68.5-86.1) had an antibody response at 1 month. The Merck rVSVΔG-ZEBOV-GP vaccine appeared to be secure and immunogenic among the many vaccinated people.

Nevertheless, fewer than one third of eligible people consented to vaccination. These information could assist information implementation selections for of cluster vaccination packages in an Ebola cluster outbreak response state of affairs

Design of Fusion Proteins for Environment friendly and Soluble Manufacturing of Immunogenic Ebola Virus Glycoprotein in Escherichia coli.

The Ebola hemorrhagic fever brought on by Ebola virus is a particularly harmful illness, and efficient therapeutic brokers are nonetheless missing. Platforms for the environment friendly manufacturing of vaccines are essential to make sure fast response in opposition to an Ebola virus outbreak.

Ebola virus glycoprotein (EbolaGP) on the virion floor is answerable for membrane binding and virus entry, thus turning into the important thing goal for vaccine improvement. Nevertheless, heterologous expression of this protein nonetheless faces engineering challenges equivalent to low manufacturing ranges and insoluble aggregation. Right here, the authors design and evaluate numerous fusion methods, attaching nice significance to the solubility-enhancing impact, and tag elimination course of.

It’s discovered {that a} C-terminal intein-based tag vastly enhances the solubility of EbolaGP and permits one-step chromatographic purification of the untagged EbolaGP by means of thiol-catalyzed self-cleavage. The purified untagged EbolaGP alone or with Freund’s adjuvant are extremely immunogenic, as confirmed in a mouse mannequin.

Consequently, the current examine places ahead a brand new technique for the environment friendly and soluble expression of untagged immunogenic EbolaGP. The intein-based protein fusion method could also be of significance for the large-scale manufacturing of the Ebola virus subunit vaccine.