Improvement of a preventive vaccine for Ebola virus an infection in primates.

Outbreaks of haemorrhagic fever brought on by the Ebola virus are related to excessive mortality charges which are a distinguishing function of this human pathogen. The best lethality is related to the Zaire subtype, one among 4 strains recognized so far. Its speedy development permits little alternative to develop pure immunity, and there’s presently no efficient anti-viral remedy.

Subsequently, vaccination affords a promising intervention to forestall an infection and restrict unfold. Right here we describe a extremely efficient vaccine technique for Ebola virus an infection in non-human primates. A mix of DNA immunization and boosting with adenoviral vectors that encode viral proteins generated mobile and humoral immunity in cynomolgus macaques.

Problem with a deadly dose of the extremely pathogenic, wild-type, 1976 Mayinga pressure of Ebola Zaire virus resulted in uniform an infection in controls, who progressed to a moribund state and demise in lower than one week. In distinction, all vaccinated animals have been asymptomatic for greater than six months, with no detectable virus after the preliminary problem. These findings reveal that it’s potential to develop a preventive vaccine towards Ebola virus an infection in primates.

Recombinant (sf9) Zaire Ebola virus glycoprotein/GP (Mayinga 1976, 1-650aa, his-tag at CT, >95%), low endotoxin
EVGP31-R-1000	Alpha Diagnostics	1000 ug	Ask for price

Recombinant (HEK) Zaire Ebola virus glycoprotein RBD domain (Mayinga 1976, 1-308 aa, His-tag, >95%, low endotoxin)
EVRB14-R-10	Alpha Diagnostics	10 ug	EUR 416.4

Recombinant (HEK) Zaire Ebola virus glycoprotein RBD domain (Mayinga 1976, 1-308 aa, His-tag, >95%, low endotoxin)
EVRB14-R-100	Alpha Diagnostics	100 ug	EUR 2617.2

Zaire Ebola virus Glycoprotein (GP), T cell epitope (577-584) (Thr-Glu-Leu-Arg-Thr-Phe-Ser-Ile) (MW: 966.1)
SP-89925-1	Alpha Diagnostics	1 mg	Ask for price

Biotin-Recombinant (sf9) Zaire Ebola virus glycoprotein/GP (Mayinga 1976, 1-650aa, his-tag at CT, >95%), low endotoxin
EVGP31-BTN	Alpha Diagnostics	10 ug	Ask for price

Recombinant (sf9) Zaire Ebola virus glycoprotein (GIN/2014/Kissidougou-C15, 1-650aa, his-tag at CT, >95%), low endotoxin
EVGP20-R-10	Alpha Diagnostics	10 ug	EUR 416.4

Recombinant (sf9) Zaire Ebola virus glycoprotein (GIN/2014/Kissidougou-C15, 1-650aa, his-tag at CT, >95%), low endotoxin
EVGP20-R-100	Alpha Diagnostics	100 ug	EUR 2617.2

Recombinant (HEK) Zaire Ebola virus glycoprotein (GIN/2014/Kissidougou-C15, 1-650aa, his-tag at CT, >95%), low endotoxin
EVGP21-R-10	Alpha Diagnostics	10 ug	EUR 416.4

Recombinant (HEK) Zaire Ebola virus glycoprotein (GIN/2014/Kissidougou-C15, 1-650aa, his-tag at CT, >95%), low endotoxin
EVGP21-R-100	Alpha Diagnostics	100 ug	EUR 2617.2

Recombinant (HEK) Zaire Ebola virus glycoprotein 1 (GIN/2014/Kissidougou-C15, GP1, 1-501aa, his-tag, >95%), low endotoxin
EVGP33-R-10	Alpha Diagnostics	10 ug	EUR 416.4

Recombinant (HEK) Zaire Ebola virus glycoprotein 1 (GIN/2014/Kissidougou-C15, GP1, 1-501aa, his-tag, >95%), low endotoxin
EVGP33-R-100	Alpha Diagnostics	100 ug	EUR 2617.2

Biotin-Recombinant (HEK) Zaire Ebola virus glycoprotein RBD domain (Mayinga 1976, 1-308 aa, His-tag, >95%, low endotoxin)
EVRB14-BTN	Alpha Diagnostics	100 ul	Ask for price

Recombinant (HEK) Zaire Ebola virus glycoprotein 2 (GP2, GIN/2014/Kissidougou-C15, GP2, 502-650aa, mFc-tag, >95%), low endotoxin
EVGP32-R-10	Alpha Diagnostics	10 ug	EUR 416.4

Recombinant (HEK) Zaire Ebola virus glycoprotein 2 (GP2, GIN/2014/Kissidougou-C15, GP2, 502-650aa, mFc-tag, >95%), low endotoxin
EVGP32-R-100	Alpha Diagnostics	100 ug	EUR 2617.2

Recombinant (HEK) Zaire Ebola virus glycoprotein RBD domain (GIN/2014/Kissidougou-C15, 1-308aa, his-tag, >95%, low endotoxin)
EVRB11-R-10	Alpha Diagnostics	10 ug	EUR 416.4

Recombinant (HEK) Zaire Ebola virus glycoprotein RBD domain (GIN/2014/Kissidougou-C15, 1-308aa, his-tag, >95%, low endotoxin)
EVRB11-R-100	Alpha Diagnostics	100 ug	EUR 2617.2

Recombinant (sf9) Zaire Ebola virus glycoprotein 1 (GIN/2014/Kissidougou-C15, GP1, 1-501aa, his-tag at CT, >95%), Low endotoxin
EVGP18-R-10	Alpha Diagnostics	10 ug	EUR 416.4

Recombinant (sf9) Zaire Ebola virus glycoprotein 1 (GIN/2014/Kissidougou-C15, GP1, 1-501aa, his-tag at CT, >95%), Low endotoxin
EVGP18-R-100	Alpha Diagnostics	100 ug	EUR 2617.2

Recombinant (HEK) Zaire Ebola virus secreted glycoprotein (sGP, Mayinga/76, 33--364aa, Flag-myc-his-tag, >95%), low endotoxin
EVGP23-R-10	Alpha Diagnostics	10 ug	EUR 416.4

Recombinant (HEK) Zaire Ebola virus secreted glycoprotein (sGP, Mayinga/76, 33--364aa, Flag-myc-his-tag, >95%), low endotoxin
EVGP23-R-100	Alpha Diagnostics	100 ug	EUR 416.4

Biotin-Recombinant (HEK) Zaire Ebola virus glycoprotein RBD domain (GIN/2014/Kissidougou-C15, 1-308aa, his-tag, >95%, low endotoxin)
EVRB11-BTN	Alpha Diagnostics	100 ul	Ask for price

Zaire ebolavirus Envelope glycoprotein (GP)
1-CSB-YP310843ZAA	Cusabio	EUR 814.80 EUR 402.00 EUR 2606.40 EUR 1261.20 EUR 1730.40 EUR 522.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Zaire ebolavirus Envelope glycoprotein(GP),partial expressed in Yeast

Zaire ebolavirus Envelope glycoprotein (GP)
1-CSB-EP311249ZAT	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Zaire ebolavirus Envelope glycoprotein(GP),partial expressed in E.coli

Monkey/Chimp Anti-Zaire Ebola virus glycoprotein (GP) IgG positive control
AE-320650-PC	Alpha Diagnostics	2 ml	EUR 270

Zaire ebolavirus Pre-small/secreted glycoprotein (GP)
1-CSB-EP350772ZAT	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Zaire ebolavirus Pre-small/secreted glycoprotein(GP) expressed in E.coli

Zaire ebolavirus Pre-small/secreted glycoprotein (GP)
1-CSB-YP308957ZAA	Cusabio	EUR 814.80 EUR 402.00 EUR 2606.40 EUR 1261.20 EUR 1730.40 EUR 522.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Zaire ebolavirus Pre-small/secreted glycoprotein(GP) expressed in Yeast

Zaire ebolavirus Pre-small/secreted glycoprotein (GP)
1-CSB-YP350772ZAT	Cusabio	EUR 814.80 EUR 402.00 EUR 2606.40 EUR 1261.20 EUR 1730.40 EUR 522.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Zaire ebolavirus Pre-small/secreted glycoprotein(GP) expressed in Yeast

Zaire ebolavirus Pre-small/secreted glycoprotein (GP)
1-CSB-EP308957ZAA	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Zaire ebolavirus Pre-small/secreted glycoprotein(GP) expressed in E.coli

Zaire ebolavirus Super small secreted glycoprotein (GP)
1-CSB-EP314099ZAT	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Zaire ebolavirus Super small secreted glycoprotein(GP) expressed in E.coli

Goat Anti-Zaire Ebola virus (Mayinga) glycoprotein (GP1-637aa) IgG, purified
EVGP22-A	Alpha Diagnostics	100 ul	EUR 578.4

Horse Anti-Zaire Ebola virus glycoprotein IgG ELISA Kit, 96 tests, Quantitative
AE-320690-1	Alpha Diagnostics	1 Kit	EUR 1220.4

Mouse Monoclonal Anti-Anti-Zaire Ebola virus glycoprotein (EBOV GP) IgG, purified
EVGP20-M	Alpha Diagnostics	100 ul	EUR 578.4

Rabbit Anti-Zaire Ebola virus glycoprotein (Mayinga 1976, 1-650aa) IgG, aff purified
EVGP25-A	Alpha Diagnostics	100 ul	EUR 578.4

Mouse Anti-Zaire Ebola virus glycoprotein (GP) IgG ELISA Kit, 96 tests, Quantitative
AE-320600-1	Alpha Diagnostics	1 kit	EUR 1074

Mouse Anti-Zaire Ebola virus glycoprotein (GP) IgM ELISA Kit, 96 tests, Quantitative
AE-320610-1	Alpha Diagnostics	1 kit	EUR 1074

Human Anti-Zaire Ebola virus glycoprotein (GP) IgG ELISA Kit, 96 tests, Quantitative
AE-320620-1	Alpha Diagnostics	1 Kit	EUR 1074

Human Anti-Zaire Ebola virus glycoprotein (GP) IgM ELISA Kit, 96 tests, Quantitative
AE-320630-1	Alpha Diagnostics	1 Kit	EUR 1074

Rabbit Anti-Zaire Ebola virus glycoprotein (GP) IgG ELISA Kit, 96 tests, Quantitative
AE-320640-1	Alpha Diagnostics	1 kit	EUR 1074

Human Anti-Zaire Ebola virus glycoprotein (GP) IgG ELISA Kit, 5x96 tests, Quantitative
AE-320620-5	Alpha Diagnostics	1 pk	EUR 4882.8

Human Anti-Zaire Ebola virus glycoprotein (GP) IgM ELISA Kit, 5x96 tests, Quantitative
AE-320630-5	Alpha Diagnostics	1 pk	EUR 4531.2

Guinea pig Anti-Zaire Ebola virus glycoprotein IgG ELISA Kit, 96 tests, Quantitative
AE-320695-1	Alpha Diagnostics	1 Kit	EUR 1220.4

Rabbit Anti-Zaire Ebola virus glycoprotein (Mayinga/GP, 1-676aa/DNA vaccine) IgG, purified
EVGP15-A	Alpha Diagnostics	100 ul	EUR 578.4

Rabbit Anti-Zaire Ebola virus glycoprotein (Mayinga/GP 1-652aa/DNA vaccine) IgG, purified
EVGP16-A	Alpha Diagnostics	100 ul	EUR 578.4

Monkey/Chimp Anti-Zaire Ebola virus glycoprotein (GP) IgG ELISA Kit, 96 tests, Quantitative
AE-320650-1	Alpha Diagnostics	1 kit	EUR 1074

Monkey/Chimp Anti-Zaire Ebola virus glycoprotein (GP) IgM ELISA Kit, 96 tests, Quantitative
AE-320660-1	Alpha Diagnostics	1 kit	EUR 1074

Rabbit Anti-Zaire Ebola virus glycoprotein 1 (Sudan/Gulu, SUDV GP1/1-320aa) IgG, purified
SVGP31-A	Alpha Diagnostics	100 ul	EUR 578.4

Rabbit Anti-Zaire Ebola virus glycoprotein (Cote dIvoire/GP, 1-672aa/DNA vaccine) IgG, purified
EVGP24-A	Alpha Diagnostics	100 ul	EUR 578.4

Mouse Monoclonal Anti-Sudan Ebola virus glycoprotein (SUDV GP) IgG, purified
SVGP22-M	Alpha Diagnostics	100 ul	EUR 578.4

Rabbit Anti-Sudan Ebola virus glycoprotein (sf9, SUDV GP 1-637aa) IgG purified
SVGP21-A	Alpha Diagnostics	100 ul	EUR 578.4

QPCR Kit RNA Zaire ebola virus
MOL6386	Scientific Laboratory Supplies	EACH	EUR 1045.79

Mouse Anti-Sudan Ebola virus glycoprotein (GP) IgG ELISA Kit, 96 tests, Quantitative
AE-321600-1	Alpha Diagnostics	1 kit	EUR 1074

Mouse Anti-Sudan Ebola virus glycoprotein (GP) IgM ELISA Kit, 96 tests, Quantitative
AE-321610-1	Alpha Diagnostics	1 kit	EUR 1074

Human Anti-Sudan Ebola virus glycoprotein (GP) IgG ELISA Kit, 96 tests, Quantitative
AE-321620-1	Alpha Diagnostics	1 Kit	EUR 1074

Human Anti-Sudan Ebola virus glycoprotein (GP) IgM ELISA Kit, 96 tests, Quantitative
AE-321630-1	Alpha Diagnostics	1 Kit	EUR 1074

Guinea pig Anti-Sudan Ebola virus glycoprotein IgG ELISA Kit, 96 tests, Quantitative
AE-321695-1	Alpha Diagnostics	1 Kit	EUR 1220.4

Guinea pig Anti-Reston Ebola virus glycoprotein IgG ELISA Kit, 96 tests, Quantitative
AE-324695-1	Alpha Diagnostics	1 Kit	EUR 1220.4

Guinea pig Anti-Marburg Ebola virus glycoprotein IgG ELISA Kit, 96 tests, Quantitative
AE-322695-1	Alpha Diagnostics	1 Kit	EUR 1220.4

Monkey/Chimp Anti-Sudan Ebola virus glycoprotein (GP) IgG ELISA Kit, 96 tests, Quantitative
AE-321650-1	Alpha Diagnostics	1 kit	EUR 1074

Monkey/Chimp Anti-Sudan Ebola virus glycoprotein (GP) IgM ELISA Kit, 96 tests, Quantitative
AE-321660-1	Alpha Diagnostics	1 kit	EUR 1074

Human Anti-Reston Ebola virus glycoprotein (REBOV GP) IgG ELISA Kit, 96 tests, Quantitative
AE-323620-1	Alpha Diagnostics	1 Kit	EUR 1074

Monkey Anti-Reston Ebola virus glycoprotein (REBOV GP) IgG ELISA Kit, 96 tests, Quantitative
AE-323630-1	Alpha Diagnostics	1 Kit	EUR 1074

G. Pig Anti-Reston Ebola virus glycoprotein (REBOVGP) IgG ELISA Kit, 96 tests, Quantitative
AE-323650-1	Alpha Diagnostics	1 Kit	EUR 1074

Guinea pig Anti-Bundibugyio Ebola virus glycoprotein IgG ELISA Kit, 96 tests, Quantitative
AE-323695-1	Alpha Diagnostics	1 Kit	EUR 1220.4

Recombinant (sf9) Bundibugyo Ebola virus glycoprotein/GP (Uganda 2007,1-501aa, His-tag, >95%) purified
BVGP45-R-10	Alpha Diagnostics	10 ug	EUR 416.4

Recombinant (sf9) Bundibugyo Ebola virus glycoprotein/GP (Uganda 2007,1-501aa, His-tag, >95%) purified
BVGP45-R-100	Alpha Diagnostics	100 ug	EUR 2617.2

TruStrip RDT Human Anti-Zaire Ebola virus glycoprotein (GP) antibody rapid test (visual results in 2-10 min), 10 cassettes/pk
AE-320625-RDT	Alpha Diagnostics	1 pk	EUR 351.6

Recombinant (HEK) Sudan Ebola virus glycoprotein 1 (SUDV GP1/Uganda, 1-501aa, his-tag, >95%), low endotoxin
SVGP28-R-10	Alpha Diagnostics	10 ug	EUR 416.4

Recombinant (HEK) Sudan Ebola virus glycoprotein 1 (SUDV GP1/Uganda, 1-501aa, his-tag, >95%), low endotoxin
SVGP28-R-100	Alpha Diagnostics	100 ug	EUR 2617.2

Human Monoclonal Anti-Falvivirus (Ebola and Marburg virus glycoprotein, GP) IgG (Neutralzing)
EVGP26-M	Alpha Diagnostics	100 ul	EUR 578.4

Rabbit Anti-Ebola-Mayinga, Zaire soluble glycoprotein peptide antiserum
SGPZ15-S	Alpha Diagnostics	100 ul	Ask for price

Recombinant Ebola Virus Ebola Zaire GP Protein, His, E.coli-1mg
QP11727-1mg	EnQuireBio	1mg	EUR 1513.2

Recombinant Ebola Virus Ebola Zaire GP Protein, His, E.coli-100ug
QP11727-100ug	EnQuireBio	100ug	EUR 261.6

Recombinant Ebola Virus Ebola Zaire GP Protein, His, E.coli-500ug
QP11727-500ug	EnQuireBio	500ug	EUR 795.6

Containment of extremely deadly Ebola virus outbreaks poses a critical public well being problem. Though an experimental vaccine has efficiently protected non-human primates towards illness, greater than six months was required to finish the immunizations, making it impractical to restrict an acute epidemic.

Right here, we report the event of accelerated vaccination towards Ebola virus in non-human primates. The antibody response to immunization with an adenoviral (ADV) vector encoding the Ebola glycoprotein (GP) was induced extra quickly than with DNA priming and ADV boosting, however it was of decrease magnitude.

To find out whether or not this earlier immune response might nonetheless shield towards illness, cynomolgus macaques have been challenged with Ebola virus after vaccination with ADV-GP and nucleoprotein (NP) vectors. Safety was extremely efficient and correlated with the technology of Ebola-specific CD8(+) T-cell and antibody responses.

Even when animals have been immunized as soon as with ADV-GP/NP and challenged 28 days later, they remained proof against problem with both low or excessive doses of virus. This accelerated vaccine offers an intervention that will assist to restrict the epidemic unfold of Ebola, and is relevant to different viruses.

Ebola virus (EBOV) causes acute hemorrhagic fever that’s deadly in as much as 90% of circumstances in each people and nonhuman primates. No vaccines or therapies can be found for human use. We evaluated the consequences in nonhuman primates of vaccine methods that had protected mice or guinea pigs from deadly EBOV an infection.

The next immunogens have been used: RNA replicon particles derived from an attenuated pressure of Venezuelan equine encephalitis virus (VEEV) expressing EBOV glycoprotein and nucleoprotein; recombinant Vaccinia virus expressing EBOV glycoprotein; liposomes containing lipid A and inactivated EBOV; and a concentrated, inactivated whole-virion preparation.

None of those methods efficiently protected nonhuman primates from strong problem with EBOV. The illness noticed in primates differed from that in rodents, suggesting that rodent fashions of EBOV might not predict the efficacy of candidate vaccines in primates and that safety of primates might require completely different mechanisms